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Application of berberine to preparing drug for treating B cell lymphoma

A technology for preparing drugs and B cells, which is applied in the application field of drugs to achieve a significant inhibitory effect and the effect of inhibiting the growth of lymphoma

Active Publication Date: 2015-07-01
RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on the role of berberine in the treatment of B-cell lymphoma, especially DLBCL and Burkitt lymphoma

Method used

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  • Application of berberine to preparing drug for treating B cell lymphoma

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0017] The specific implementation manners provided by the present invention will be described in detail below.

[0018] 1. Materials and methods

[0019] 1.1 Reagents

[0020] Berberine was purchased from Sigma-Aldrich Company.

[0021] 1.2 Cell culture

[0022] Human Burkitt lymphoma cell lines Raji and Namalwa, and DLBCL lymphoma cell line SU-DHL-4 were purchased from the American Type Culture Collection at 3×10 5 / ml density inoculated in RPMI1640 medium with 10% heat extinguished fetal bovine serum, placed at 37°C, 5% CO 2 , cultured in an incubator with 95% humidity, and the medium was changed every 48 hours, and the cells in the logarithmic growth phase were used in the test.

[0023] 1.3 Detection of cell proliferation toxicity

[0024] Set the density to 3×10 5 / ml logarithmic growth phase cell suspension was seeded in 96-well plate. The final concentrations of Berberine were set to 1, 5, 10, 50, and 100 μM to treat the cells, and a blank group without drug add...

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Abstract

The invention relates to an application of berberine to preparing a drug for treating B cell lymphoma. Three B cell lymphoma cell strains are applied to perform in-vivo experiment, and a B cell lymphoma mouse model is established to perform in-vitro experiment, so that the berberine is proved to have very remarkable effects of restraining B cell lymphoma cell strains from multiplication and restraining cancer-bearing mouse lymphoma from growth; the berberine can be used as an effective drug for treating the B cell lymphoma, and the drug safety is verified for a long time, so that a novel scheme is provided for treating clinical B cell lymphoma.

Description

technical field [0001] The invention relates to a new application of berberine, in particular to the application of berberine in the preparation of medicines for treating B-cell lymphoma. Background technique [0002] Berberine (C 20 h 18 ClNO 4 ) is an ancient traditional Chinese medicine, which can resist a variety of pathogenic microorganisms. It is mainly used clinically to treat bacillary dysentery and gastroenteritis. More and more studies now show that berberine can improve the immune system, cardiovascular system, nervous system and other diseases to varying degrees. Sexual muscle strength, protection of ischemic brain injury, hypoglycemia, anti-inflammation, inhibition of platelet aggregation, anti-ulcer, antipyretic and other effects. Because berberine also has the advantages of mild side effects and long-term use, it is suggested that it has broad clinical application prospects. [0003] B-cell lymphoma is a solid tumor arising from B-cells. There are many ty...

Claims

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Application Information

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IPC IPC(8): A61K31/4375A61P35/00
Inventor 赵维莅王黎郑重薛雯张翼飞宁光
Owner RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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