Raman spectrum detection method for detecting sample in container

Abstract

An embodiment of the present invention provides a Raman spectroscopic detection method for detecting a sample in a vessel, comprising the steps of: (a) measuring a Raman spectrum of the vessel to obtain a first Raman spectroscopic signal; (b) measuring a Raman spectrum of the sample through the vessel to obtain a second Raman spectroscopic signal; (c) removing an interference caused by the Raman spectrum of the vessel from the second Raman spectroscopic signal on basis of the first Raman spectroscopic signal to obtain a third Raman spectroscopic signal of the sample itself; and (d) identifying the sample on basis of the third Raman spectroscopic signal. By means of the above method, the Raman spectrum of the sample in the vessel may be detected correctly so as to identify the sample to be detected efficiently.

Description

technical field [0001] The invention relates to the field of Raman spectrum detection, in particular to a method for detecting samples in a container by using Raman spectrum technology. Background technique [0002] Raman spectroscopy is a kind of molecular vibration spectrum, which can reflect the fingerprint characteristics of molecules and can be used for the detection of substances. Raman spectrum detection detects and identifies substances by detecting the Raman spectrum generated by the Raman scattering effect of the analyte on the excitation light. Raman spectroscopy detection method has been widely used in liquid security inspection, jewelry detection, explosive detection, drug detection, drug detection and other fields. [0003] One problem encountered in the detection of substances by the Raman spectroscopy detection method is the detection of the sample in the container. Since the sample to be tested sometimes needs to be housed or even packaged in a container, ...

AI Technical Summary

Problems solved by technology

For the sample contained in the container, in order to collect its Raman spectrum, the excitation light must first pass through the container, and the container of plastic, glass and other materials will generate some signals after being excited by light. , which will lead to a large difference between the collected spectrum and the Raman spectrum of the measured sample itself, which will lead to the inability to accurately identify many substances

At present, no good solution has been found for the impact of the container, and the sample to be tested often has to be taken out of the container for direct detection, which is very inconvenient

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