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Detection apparatus and method for content of chlorophyll

A technology of chlorophyll content and detection method, which is applied in the measurement of color/spectral characteristics, etc., can solve the problems of interference, interference and high environmental requirements of detection results, and achieve the effects of fast analysis speed, no chemical pollution, and wide measurement range.

Active Publication Date: 2015-07-15
CHINA AGRI UNIV
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Problems solved by technology

Since the fluorescence intensity generated by excitation is very weak, this detection method has very high environmental requirements in actual detection and is easily interfered by ambient light.
At the same time, due to the uncertainty of impurities in water samples, the test results will also be interfered to a certain extent.

Method used

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  • Detection apparatus and method for content of chlorophyll
  • Detection apparatus and method for content of chlorophyll
  • Detection apparatus and method for content of chlorophyll

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Embodiment Construction

[0043] The specific embodiments of the invention will be further described below in conjunction with the accompanying drawings. The following examples are only used to illustrate the technical solution of the present invention more clearly, but not to limit the protection scope of the present invention.

[0044] figure 1 A schematic flow diagram showing a method for detecting chlorophyll content provided by an embodiment of the present invention, as figure 1 As shown, the method includes the following steps:

[0045] 101. Using different concentrations of chlorophyll solutions to establish a chlorophyll detection model;

[0046] 102. Use two wavelengths of incident light to detect the sample to be tested, and obtain the absorbance of chlorophyll of the sample to be tested; the two wavelengths are 663 nm and 645 nm.

[0047] 103. Substitute the absorbance of chlorophyll of the sample to be tested into the chlorophyll detection model to obtain the concentration of chlorophyll...

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Abstract

The invention discloses a detection apparatus and method for the content of chlorophyll. The method comprises the following steps: establishing a chlorophyll detection model by using chlorophyll solutions with different concentrations; detecting a to-be-detected sample by using incident light of two wavelengths so as to acquire the absorbance of chlorophyll in the to-be-detected sample; and substituting the absorbance of chlorophyll in the to-be-detected sample into the chlorophyll detection model so as to obtain the concentration of chlorophyll in the to-be-detected sample. According to the invention, light with wavelengths of 645 nm and 663 nm is used to irradiate the sample, the value of absorbance at corresponding wavelength can be obtained by measuring the intensity of transmitted light via a photoelectric sensor, so the relative concentration value of chlorophyll can be calculated. The detection apparatus and method have the main advantages of a fast analysis speed, a wide measurement range, simple preparation of the sample, no consumption of the sample, no chemical pollution, etc.

Description

technical field [0001] The invention relates to the technical field of spectrum detection, in particular to a detection device and method for chlorophyll content. Background technique [0002] As the saying goes: "Fish first raises water". In order to achieve the success of aquaculture, the most critical thing is to control the water quality in place. The key to water quality regulation in aquaculture is: dissolved oxygen, transparency, pH, etc. Among them, dissolved oxygen is the life element of aquaculture animals, and the content of chlorophyll determines the dissolved oxygen in water. Chlorophyll is an important component of algae, and the content of chlorophyll can also reflect the water quality to a certain extent. [0003] The method of measuring the chlorophyll content of water body with spectrophotometer 721 belongs to the method of sampling analysis and measurement. The usual practice is to take water samples on site and bring them back to the laboratory for anal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
Inventor 李民赞糜修尘郑立华张瑶张琴孙红
Owner CHINA AGRI UNIV
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