Application of platycarya strobilacea infructescence extract
A technology for the fruit and extract of Fructus Fructus, applied in the application field of the Fructus Fructus Fructus fruit sequence extract, can solve problems such as unseen research, and achieve the effect of simple extraction process
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Embodiment 1
[0011] Dry in the shade until the water content is 13.2wt% of 2000g of the fragrant inflorescence raw material, remove impurities, crush to 3-5mm, add 12L of pure aqueous solution and extract in a water bath at 80°C for 1 hour, filter the extract for solid-liquid separation, and repeat the extraction for two The second time, the waste residue was discarded, and the extracts were combined. The extract is cooled and left to settle overnight, and the precipitate is discarded. The supernatant is concentrated in vacuum and freeze-dried to obtain the Huaxiang inflorescence extract, and the tannin content is greater than 76%.
Embodiment 2
[0013] According to the addition amount of 0.5wt.%, 1.0wt.% and 2.0wt.%, the Huaxiang fruit sequence extract obtained in Example 1 was added to the commercially available grouper powder feed respectively, and after mixing evenly, fish oil and water were added to carry out Modulation (the amount of water added to the feed is to add 900-100g water for every 2.5kg feed, and add fish oil according to the 5% ratio of the feed mass fraction), adopt a twin-screw feed pelletizer to make 1.5mm hard pellet feed, carry out breeding experiments, and investigate the feed Effects of additives on growth performance of blue grouper.
[0014] Two repetitions were set up for each experimental group, and the experimental fry were randomly assigned to 8 200L rectangular blue plastic water tanks with running water, and 60 fish were stocked in each water tank, and the experimental feed was fed twice a day at 8:00 and 16:00 , every time you are full, and remove the feces in time. During the breedin...
Embodiment 3
[0019] In order to study the pathogenicity of Vibrio parahaemolyticus to blue grouper, 17 grouper were randomly selected and divided into 4 concentration groups, and each group was injected with different concentrations of bacterial solution (5 μL / g ) to determine its half-lethal concentration. The results showed that grouper injected with OD600 value of 0.17 Vibrio parahaemolyticus would not be lethal, so the group injected with 35-fold dilution was used as the control group. After 72 hours, observe and determine the median lethal concentration, that is, OD600 is about 0.6-2.0.
[0020] Add the extract of Huaxiang fruit sequence to the feed at a ratio of 0.5% to raise the blue grouper, and set up a control group (no addition group) at the same time. After the feeding experiment was over, 20 healthy blue grouper with complete body surface were taken from the two experimental groups for the challenge injection experiment of Vibrio parahaemolyticus. Resuspend Vibrio parahaemol...
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