Image sequence and evaluation method and system for structured illumination microscopy
A pattern, space pattern technology, applied in the field of microscope system, can solve the problem of partial tilt, no provision, different height and reflectivity, etc.
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[0059] figure 1 An apparatus 1 for forming a three-dimensional (3D) height map of a specimen 8 is shown. The device 1 comprises a light source 2 constructed and arranged to generate a light beam 3 . In the path of the light beam 3 a spatial light modulator 4, a lens 5 and a beam splitter 6 are arranged. The device 1 also comprises an objective lens 7 adapted to receive and direct the beam of light 3, and a support 20 for carrying a specimen 8 whose upper surface 9 is to be determined with a height map. The device 1 is also equipped with a second lens 10, and a two-dimensional photodetector 11 (such as a camera) comprising an array of photodetector elements, each photodetector element being adapted to receive incident light of the light beam 3 The intensity is transformed into a signal. The signal is sent to a processing unit 12, preferably formed by a computer or computer system programmed to perform control of the device 1 and processing of signal data to provide said hei...
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