Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Ergothioneine production through metabolic engineering

An ergothioneine and gene technology, applied in the field of ergothioneine production through metabolic engineering, can solve the problems that EgtE enzyme cannot be overexpressed, EgtB activity is low, and production is limited.

Active Publication Date: 2015-08-19
刘平华 +2
View PDF7 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are three major obstacles hindering the use of currently known ergothioneine biosynthetic pathways produced by fermentation: (1) the EgtE enzyme cannot be overexpressed; (2) the activity of EgtB is low; (3) ergothioneine and glutathione Potential competition between glycides for y-Glu-Cys would limit yield

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Ergothioneine production through metabolic engineering
  • Ergothioneine production through metabolic engineering
  • Ergothioneine production through metabolic engineering

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0307] Example 1: Production of Ergothioneine by Metabolic Engineering (In Vitro Enzymatic Means)

[0308] Identification of a gene cluster for ergothioneine biosynthesis. Ergothioneine and ovothiol (5&7, figure 1 ) are metabolites of two thiol-imidazoles isolated from ergot by Tanret in 1909 [1]. The presence of ergothioneine-specific transporters in humans [2] allows us to enrich ergothioneine from the diet, reaching millimolar concentrations in several organs such as the liver, kidney, central nervous system and erythrocytes[ 3]. Unlike other thiols, the balance between the thiolate and thione forms of the thiol-imidazole side chain in ergothioneine and ovothiol is predominantly in favor of the thione form (e.g. figure 1 5) in [3f, 4], which makes them more stable in oxidation than other thiol mimics (eg, glutathione) [4a]. This unique redox property of ergothioneine [4a, 5] enables it to protect hemoglobin from oxidation in erythrocytes and avoid cataract formation in ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present disclosure relates to the production of ergothioneine through either in vitro enzymatic transformations or fermentations using microbials created by metabolic engineering. Also disclosed are transformed cells useful in such methods and ergothioneine produced by such methods. Transformed cells of the disclosure are capable of converting histidine and cysteine or hercynine and cysteine into ergothioneine in greater efficiency than the untransformed wild-type cells.

Description

[0001] related application [0002] This application claims priority and the benefit of US Patent Provisional Application No. 61 / 740,829, filed December 21, 2012. The entire content of this application is incorporated herein by reference in its entirety. [0003] Government funding [0004] This invention was made under government grant GM093903 awarded by the National Institutes of Health and CHE-0748504 awarded by the National Science Foundation. The US Government has certain rights in this invention. technical field [0005] The present disclosure generally relates to the production of ergothioneine by cell-free enzymatic conversion or by fermentation using microorganisms produced by metabolic engineering. Background technique [0006] Ergothioneine is an amino acid containing a thiol group isolated from ergot by Tanret in 1909. Its unique redox properties make it one of the best natural antioxidants. Furthermore, since it is specifically enriched from food by many h...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/04C12N15/52C12N15/70
CPCC12P13/04C12N15/52C07D233/42C12P17/10
Inventor 刘平华宋恒胡文
Owner 刘平华
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com