107 results about "Wild type cell" patented technology
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Wild type barm cells contain VRP1 cistron that encodes Verprolin protein. VRP1 protein is the barm ( S. cerevisiae ) ortholog of human Wiskott-Aldrich syndrome protein ( WASP ) -interacting protein ( WIP ) .
The invention discloses a gRNA subjected to wild typeT cell TCR alpha chain knockout and a method. The sequence of the gRNA is shown as SEQ ID NO:1. By using a CRISPR / Cas 9 technology, the gRNA and the CRISPR / Cas9 perform co-infection on T cells; the wild typeT cell TCR alpha chain knockout is performed; the T cells lack of wild typeT cell TCR alpha chains are built; the gRNA can be used for CAR-T or TCR-T cellular immunity treatment. The gRNA has high knockout rate; the preparation method is relatively simple and easy; the T cells lack of wild type T cell TCR alpha chains can be fast and efficiently provided for clinics.
The present invention provides a recombinant gram-negative bacterial cell comprising a mutant spr gene encoding a spr protein having a mutation at one or more amino acids selected from D133, H145, H157, N31, R62, I70, Q73, C94, S95, V98, Q99, R100, L108, Y115, V135, L136, G140, R144 and G147 and wherein the cell has reduced Tsp protein activity compared to a wild-type cell.