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A kind of polypeptide extracted from the venom of fire jellyfish cnidaria and its application

A fire jellyfish thorn and venom technology, which is applied in the field of peptides, can solve the problems of difficulty in separation and purification of jellyfish toxins and imitation of toxin separation and purification, and achieve the effects of inhibiting the growth of HUVEC, fewer separation steps and simple operation.

Inactive Publication Date: 2018-08-03
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Different types of jellyfish have great differences in their physiological structure, toxin composition and activity contained in their bodies, which makes it difficult to follow the separation and purification of toxins from different types of jellyfish, and can only be obtained through complicated experiments.
Therefore, the separation and purification of jellyfish toxins is very difficult

Method used

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  • A kind of polypeptide extracted from the venom of fire jellyfish cnidaria and its application
  • A kind of polypeptide extracted from the venom of fire jellyfish cnidaria and its application
  • A kind of polypeptide extracted from the venom of fire jellyfish cnidaria and its application

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Experimental program
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Effect test

Embodiment 1

[0042] A polypeptide molecule, the amino acid sequence of which is shown in SEQ ID No.1;

[0043] SEQ ID No. 1:

[0044]

[0045]

Embodiment 2

[0047] The extraction of the polypeptide of the present invention:

[0048] Step 1. Take the tentacles from the live fire jellyfish and mix them with deionized water at 0°C in a volume ratio of 1:1. After stirring and standing still, take the precipitate and repeat it twice. Centrifuge for the first time. The centrifugation conditions are: centrifugal force 1000G, 4°C, 15min, collect the precipitate;

[0049] Step 2. Mix the precipitate obtained in step 1 with the disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution with a total phosphate concentration of 0.025mol / L and a pH of 5.5 according to the mass volume ratio of 1g: 40mL, and ultrasonically crush it for the second time. Centrifuge, the centrifugation conditions are: centrifugal force 13000G, 4°C, 2h, collect the supernatant to obtain the venom protein;

[0050] Step 3, take the venom protein obtained in step 2, dilute it 3 times with a buffer solution of disodium hydrogen phosphate-sodium dihydrogen ...

Embodiment 3

[0054] The extraction of the polypeptide of the present invention:

[0055] Step 1. Take the tentacles from the live fire jellyfish and mix them with deionized water at 4°C at a volume ratio of 1:1.5. After stirring and standing still, take the precipitate and repeat it for 4 times. Centrifuge for the first time. The centrifugation conditions are: centrifugal force 1000G, 4°C, 15min, collect the precipitate;

[0056] Step 2, mix the precipitate obtained in step 1 with the disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution with a total phosphate concentration of 0.025mol / L and a pH of 6.5 according to the mass volume ratio of 1g: 60mL, and ultrasonically crush it for the second time Centrifuge, the centrifugation conditions are: centrifugal force 13000G, 4°C, 2h, collect the supernatant to obtain the venom protein;

[0057] Step 3, take the venom protein obtained in step 2, dilute it 5 times with the disodium hydrogen phosphate-sodium dihydrogen phosphate ...

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Abstract

The invention discloses a polypeptide extracted from the venom of the fire jellyfish cnidaria, the amino acid sequence of which is shown in SEQ ID No.1. The invention also discloses a method for extracting the polypeptide: extracting the venom protein from the venom of Pyrogus jellyfish, and then sequentially using a Histrap SP strong cation exchange column and a Histrap Q strong anion exchange column to separate the polypeptide from the venom protein. The invention also discloses the application of the polypeptide in the preparation of drugs for anti-tumor-induced angiogenesis. The polypeptide of the invention can inhibit the growth of umbilical cord vein endothelial cells HUVEC and induce the apoptosis of umbilical cord vein endothelial cells HUVEC. The extraction method adopted by the invention is simple to operate, has few separation steps, and is suitable for rapid separation; the separation condition is mild, and the antitumor activity of the obtained protein can be preserved to the greatest extent.

Description

technical field [0001] The invention relates to the fields of marine biological technology and medicine, in particular to a polypeptide extracted from the nematode venom of Pyrogus jellyfish and its application. Background technique [0002] Tamoya alata Uchida is a large and highly toxic jellyfish that is only distributed in the Beibu Gulf of my country. It has long tentacles and a large number of nematocyst cells. It contains venom whose main active ingredient is protein / polypeptide. When people or animals come into contact, the nematocysts will penetrate into the skin, penetrate deep into the dermis, and release the toxins stored in the cells, causing stings. Depending on the severity of the injury, the victim will experience skin redness, swelling, tingling, bleeding, and skin ulceration immediately at the stung site. In severe cases, the victim will become weak, have fever, vomit, and even die. The reason is that the stinging cells of jellyfish tentacles contain a large...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/435C07K1/18A61K38/17A61P35/00
Inventor 屈啸声李刚夏祥华樊兰兰钟桃珍
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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