Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Drug application of polygonum cuspidatum total extract, polydatin or resveratrol

A technology of resveratrol and polydipside, which is applied in the field of medicine to achieve the effect of inhibiting the formation of zebrafish lower intestinal vein blood vessels and inhibiting the increase in value.

Inactive Publication Date: 2019-12-24
HKUST SHENZHEN RES INST
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The purpose of the present invention is to provide a pharmaceutical application of Polygonum cuspidatum total extract, polydatin or resveratrol, to solve the problem that the total extract of Polygonum cuspidatum, polydatin or resveratrol is not used in the preparation of anti-angiogenesis drugs in the prior art. drug problem

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Drug application of polygonum cuspidatum total extract, polydatin or resveratrol
  • Drug application of polygonum cuspidatum total extract, polydatin or resveratrol
  • Drug application of polygonum cuspidatum total extract, polydatin or resveratrol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1: Preparation of Polygonum cuspidatum total extract and HPLC fingerprint of Polygonum cuspidatum total extract

[0054] 1. Method

[0055] Crushed Polygonum cuspidatum medicinal materials, weighed 50g, as for the volumetric flask, soaked in 1000mL, 50% ethanol for three days, the extract was concentrated in vacuum at 60℃ to 30mL and then lyophilized in vacuo to obtain the total extract of Polygonum cuspidatum, and the lyophilized powder was stored in vacuum. .

[0056] Weigh 100 mg of the lyophilized powder, place it in a 15 mL centrifuge tube, add 4 mL, 50% methanol, sonicate for 15 minutes, and centrifuge at 1000 x g for 5 minutes to obtain the supernatant. Use 0.45 μm for the supernatant before injection Filtration through the microporous membrane, take the additional filtrate, and analyze it. The analytical instrument used is Agilent liquid phase with autosampler and binary pump, the chromatographic column is Agilent, Grace VisionHT C18 column (4.6x 250mm, 5μm),...

Embodiment 2

[0060] Example 2: Polygonum cuspidatum total extract, polydatin and resveratrol inhibit human umbilical vein endothelial cell (HUVEC) proliferation experiment

[0061] The colorimetric method of tetrazolium salt (MTT) was used to observe the positive control group Avastin group and the inhibitory effect of polydatin extract, polydatin and resveratrol on the proliferation of HUVEC at different concentrations, indicating that the total extract of Polygonum cuspidatum Polydatin, polydatin and resveratrol have a good inhibitory effect on HUVEC proliferation, and there is a concentration-dependent relationship.

[0062] 1. Method

[0063] HUVEC cells at 5.0×10 3 Cells / mL were inoculated in 96-well culture plates with 100μL per well. After cell growth reached 80% confluence, add 10ng / mL VEGF or different concentrations of Polygonum cuspidatum total extract, polydatin and resveratrol into the wells After 48 hours of action, add 10μL of MTT solution to each well, continue to incubate at 37°...

Embodiment 3

[0067] Example 3: Polygonum cuspidatum total extract, polydatin and resveratrol inhibit human umbilical vein endothelial cell (HUVEC) scratch migration experiment

[0068] 1. Method

[0069] HUVEC cells are 20×10 4 Cells / well were seeded in a 12-well plate and cultured overnight. After the cells grew to 80% confluence, use a 200μL pipette tip to scratch a lateral wound in the middle of each well and discard the medium. Wash it with PBS, add 10ng / mL human vascular endothelial cell growth factor (VEGF), 200μg / mL Aisiting, and different concentrations of Polygonum cuspidatum total extract, polydatin and resveratrol separately into the culture medium. Set up a blank experiment without adding medicine. A microscope equipped with a camera was used to photograph the changes in the coverage of the cell layer in each well after 0 and 8 hours under 50 times magnification. Then use TScratch software to digitize the recovery growth rate, and calculate the cell recovery growth rate according ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Apertureaaaaaaaaaa
Login to View More

Abstract

The invention relates to a drug application of a polygonum cuspidatum total extract, polydatin or resveratrol in preparation of a medicine capable of being combined with a vascular endothelial growthfactor to inhibit angiogenesis. The anti-angiogenesis effects of the polygonum cuspidatum total extract, polydatin and resveratrol are discovered for the first time, and the application finds the polygonum cuspidatum total extract, polydatin and resveratrol can effectively inhibit proliferation, migration and tubule formation of vascular endothelial cells for the first time, can effectively inhibit the generation of subintestinal veins of zebra fish, have anti-angiogenesis activity, can be used as an angiogenesis inhibitor, and can be used for preparing the medicine for treating and preventingneovascularization-dependent and neovascularization-related diseases such as tumors, arthritis, psoriasis, ophthalmic diseases, atherosclerosis and the like.

Description

Technical field [0001] The invention relates to the technical field of medicine, in particular to a pharmaceutical application of Polygonum cuspidatum total extract, polydatin or resveratrol. Background technique [0002] Angiogenesis is a physiological phenomenon in the human body, which plays an important role in embryonic development and tissue damage repair. Angiogenesis is a complex process involving multiple cells and multiple molecules, including endothelial cells secreting proteases to degrade basement membrane, endothelial cell proliferation and migration, formation of new capillary lumen structure, and recruiting pericytes to stabilize newly formed capillaries. The vascular network forms a mature vascular network through the remodeling and expansion of the original blood vessels. [0003] The dynamic balance between inhibiting angiogenesis molecules and promoting angiogenesis molecules is considered to be a "switch" for regulating angiogenesis. Under normal physiologica...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K36/704A61K31/7034A61K31/05A61P35/00A61P17/06A61P19/02A61P9/10A61P27/02A61P29/00
CPCA61K31/05A61K31/7034A61K36/704A61P9/10A61P17/06A61P19/02A61P27/02A61P29/00A61P35/00
Inventor 詹华强胡伟慧董婷霞段然
Owner HKUST SHENZHEN RES INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com