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Polypeptide extracted from nematocyst venom of fire medusa and application thereof

A technology of fire jellyfish thorns and venom, applied in the field of polypeptides, can solve the problems of difficulty in separation and purification of jellyfish toxins, difficulty in the separation and purification of toxins, and achieve the effect of fewer separation steps, rapid separation, and mild separation conditions

Inactive Publication Date: 2015-09-02
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Different types of jellyfish have great differences in their physiological structure, toxin composition and activity contained in their bodies, which makes it difficult to follow the separation and purification of toxins from different types of jellyfish, and can only be obtained through complicated experiments.
Therefore, the separation and purification of jellyfish toxins is very difficult

Method used

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  • Polypeptide extracted from nematocyst venom of fire medusa and application thereof
  • Polypeptide extracted from nematocyst venom of fire medusa and application thereof
  • Polypeptide extracted from nematocyst venom of fire medusa and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A polypeptide molecule, the amino acid sequence of which is shown in SEQ ID No.1;

[0043] SEQ ID No. 1:

[0044]

[0045]

Embodiment 2

[0047] The extraction of the polypeptide of the present invention:

[0048] Step 1. Take the tentacles from the live fire jellyfish and mix them with deionized water at 0°C in a volume ratio of 1:1. After stirring and standing still, take the precipitate and repeat it twice. Centrifuge for the first time. The centrifugation conditions are: centrifugal force 1000G, 4°C, 15min, collect the precipitate;

[0049] Step 2. Mix the precipitate obtained in step 1 with the disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution with a total phosphate concentration of 0.025mol / L and a pH of 5.5 according to the mass volume ratio of 1g: 40mL, and ultrasonically crush it for the second time. Centrifuge, the centrifugation conditions are: centrifugal force 13000G, 4°C, 2h, collect the supernatant to obtain the venom protein;

[0050] Step 3, take the venom protein obtained in step 2, dilute it 3 times with a buffer solution of disodium hydrogen phosphate-sodium dihydrogen ...

Embodiment 3

[0054] The extraction of the polypeptide of the present invention:

[0055] Step 1. Take the tentacles from the live fire jellyfish and mix them with deionized water at 4°C at a volume ratio of 1:1.5. After stirring and standing still, take the precipitate and repeat it for 4 times. Centrifuge for the first time. The centrifugation conditions are: centrifugal force 1000G, 4°C, 15min, collect the precipitate;

[0056] Step 2, mix the precipitate obtained in step 1 with the disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution with a total phosphate concentration of 0.025mol / L and a pH of 6.5 according to the mass volume ratio of 1g: 60mL, and ultrasonically crush it for the second time Centrifuge, the centrifugation conditions are: centrifugal force 13000G, 4°C, 2h, collect the supernatant to obtain the venom protein;

[0057] Step 3, take the venom protein obtained in step 2, dilute it 5 times with the disodium hydrogen phosphate-sodium dihydrogen phosphate ...

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Abstract

The present invention discloses a polypeptide extracted from the nematocyst venom of fire medusa. The amino acid sequence of the polypeptide is shown in SEQ ID No.1. The invention also discloses a method for extracting the above polypeptide. According to the method, venom proteins are extracted from the nematocyst venom of fire medusa, and then the polypeptide is separated from the venom proteins successively by means of a Histrap SP strong cation exchange column and a Histrap Q strong anion exchange column. The invention also discloses the application of the above polypeptide in preparing anti-tumor induced angiogenesis drugs. By means of the above polypeptide, the growth of human umbilical vein endothelial cells (HUVEC) is inhibited, and the apoptosis of HUVEC is induced. Meanwhile, the above extracting method is simple to operate and fewer in separating step, thus being applicable to rapid separation. Moreover, the polypeptide is mild in separation condition, so that the anti-tumor activity of obtained proteins can be maintained maximally.

Description

technical field [0001] The invention relates to the fields of marine biological technology and medicine, in particular to a polypeptide extracted from the nematode venom of Pyrogus jellyfish and its application. Background technique [0002] Tamoya alata Uchida is a large and highly toxic jellyfish that is only distributed in the Beibu Gulf of my country. It has long tentacles and a large number of nematocyst cells. It contains venom whose main active ingredient is protein / polypeptide. When people or animals come into contact, the nematocysts will penetrate into the skin, penetrate deep into the dermis, and release the toxins stored in the cells, causing stings. Depending on the severity of the injury, the victim will experience skin redness, swelling, tingling, bleeding, and skin ulceration immediately at the stung site. In severe cases, the victim will become weak, have fever, vomit, and even die. The reason is that the stinging cells of jellyfish tentacles contain a large...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C07K1/18A61K38/17A61P35/00
Inventor 屈啸声李刚夏祥华樊兰兰钟桃珍
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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