Tumor phototherapy reagent based on cell membrane coating, as well as preparation method and application of tumor phototherapy reagent
A technology of cell membranes and reagents, applied in the field of anti-tumor phototherapy reagents, can solve the problems of weakening the yield of singlet oxygen and phototherapy efficiency, and achieve the improvement of yield of singlet oxygen and fluorescence brightness, inhibition of self-quenching, good biophase capacitive effect
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Embodiment 1
[0041] (1) Firstly, using glycol chitosan (glycol chitosan) and N-hydroxysuccinimide ester-polyethylene glycol 2000-methoxyl (NHS-PEG2000-OMe) as raw materials, synthesize PEG molecules to account for chitosan The percentage of sugar repeating units is 20% of the precursor, ie chitosan-20% PEG. Weigh 40mg of NHS-PEG2000-OMe and 20mg of ethylene glycol chitosan respectively, dissolve them in PBS buffer (pH 7.4, 50mM), mix quickly and uniformly, and react overnight at room temperature in a shaker. After the reaction, it was purified by dialysis with a dialysis bag with a molecular weight cut-off of 10k for 3 days, and finally freeze-dried in a lyophilizer to make chitosan-20% PEG.
[0042] (2) Subsequently, chitosan-20% PEG, protoporphyrin (PpIX), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC) and N-hydroxysuccinyl Imine (NHS) was used as a raw material to synthesize a phototherapy agent in which PpIX molecules accounted for 4% of chitosan repeating units: chitosan-20%PEG-...
Embodiment 2
[0046] According to the method of Example 1, protoporphyrin is replaced by indocyanine green, adjust the consumption of each component, the percentage of synthetic PEG accounting for chitosan repeating unit number is 2%, and indocyanine green molecule accounts for chitosan repeating unit The percentage of the number is 0.2% of the reagent.
Embodiment 3
[0048] According to the method of Example 1, protoporphyrin is replaced by chlorin E6, the consumption of each component is adjusted, the percentage of synthetic PEG accounting for chitosan repeating unit number is 30%, and chlorin E6 molecule accounts for chitosan The percentage of repeating units is 2% of reagents.
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