Three-temperature-region channel embedded continuous flow laminated PCR (polymerase chain reaction) chip

Abstract

The invention provides a three-temperature-region channel embedded continuous flow laminated PCR (polymerase chain reaction) chip. Temperature measurement electrodes are arranged on the upper surface of an upper substrate; heating electrodes are arranged on the lower surface of a lower substrate; the upper substrate and lower substrate are bonded to form an integral body; and the reaction channels are formed by splicing grooves which are symmetrically etched on the lower surface of the upper substrate and the upper surface of the lower substrate. The substrates are divided into three different regions at certain intervals according to three different reaction regions of PCR, and the reaction solution continuously flows through the three different regions to complete the reaction; and in one reaction region, the temperature distribution uniformity is higher, and no repeated rise and fall of the temperature are needed, thereby shortening the total reaction time and saving the time. The reaction channels are symmetrically arranged on the upper and lower surfaces, so that the distance between the temperature measurement elements and the reaction solution becomes shorter, and the temperature measurement accuracy is higher. The fine hoses in the reaction channels restrict the reaction solution, thereby reducing the generation of bubbles.

Description

technical field [0001] The invention relates to a PCR chip in the field of PCR biotechnology, in particular to a continuous-flow laminated PCR chip embedded in three temperature zone channels. Background technique [0002] Polymerase chain reaction (PCR) is a molecular biology technique commonly used in the field of biological science and medicine to amplify specific DNA fragments at high speed and in large quantities. A cycle in PCR is divided into three sections, high-temperature denaturation (about 95°C), low-temperature annealing (about 55°C), and medium-temperature extension (about 70°C). On a conventional PCR instrument, a cycle takes about 3 minutes, and this A considerable part of the time is used for temperature rise and fall, and the time utilization rate is low. The main factors affecting the success or failure of PCR reaction and amplification efficiency include temperature uniformity, temperature control accuracy, and heating and cooling rate. The existing con...

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Problems solved by technology

The existing conventional PCR chip has low amplification efficiency, and the microfluidic PCR chip realized by MEMS technology has problems such as poor temperature uniformity and inaccurate temperature measurement.

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