Novel low density lipoprotein cholesterol electrochemistry immunosensor preparation and application thereof

A low-density lipoprotein and immunosensor technology is applied in the field of experimental instruments to achieve the effects of low price, favorable for industrialized production and simplified experimental operation process

Active Publication Date: 2015-09-16
NANJING UNIV OF TECH
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The deposition of low-density lipoprotein LDL is closely related to the occurrence and development of atherosclerosis. However, compared with the measurement of blood sugar content, the use of electrochemical techniques to measure cholesterol content, especially the quantitative detection of low-density lipoprotein cholesterol is very rare. Therefore, the research technology of the miniaturized cholesterol rapid tester needs to be improved and perfected urgently

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Novel low density lipoprotein cholesterol electrochemistry immunosensor preparation and application thereof
  • Novel low density lipoprotein cholesterol electrochemistry immunosensor preparation and application thereof
  • Novel low density lipoprotein cholesterol electrochemistry immunosensor preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The schematic diagram of the device structure of this embodiment is attached figure 1As shown, it mainly includes silver / silver chloride electrode; platinum wire electrode; the measurement system composed of modified ITO electrode; the sample channel-detection area system composed of PDMS and working electrode and the signal output system of electrochemical workstation.

[0042] On the 2cm*4cm rectangular ITO glass modified with amino groups, use an inkjet printer to print PDMS containing carboxyl groups, and dry at 50°C for 2-4 hours to cure and shape. The PDMS channel is 3-7mm wide and 3-6mm high. The diameter of the area is 70-95mm, and the diameter of the detection area is 40mm-65mm. A measuring device integrating sample pool and detection is prepared. Using the modified ITO glass as the working electrode, the detection area 1 first prints 5-10 layers of AuNPs-Ab that only bind LDL by an inkjet printer LDL mixed solution. Then the two detection areas print the mo...

Embodiment 2

[0045] The schematic diagram of the device structure of this embodiment is attached figure 1 As shown, it mainly includes silver / silver chloride electrode; platinum wire electrode; measurement system composed of modified ITO electrode; sample cell assembled with PDMS and working electrode and signal output system of electrochemical workstation.

[0046] Use the centrifuge system to separate the high and low density cholesterol, and only add the LDL-C sample. Determination according to the same steps as in Example 1, the LDL-C content and the free cholesteryl ester / triglyceride content can be measured and calculated respectively.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of experiment apparatus, and concretely relates to a novel low density lipoprotein cholesterol LDL-C immunosensor preparation and an application thereof. An ink-jet printing technology is employed for printing polydimethylsiloxane PDMS on indium tin oxide glass ITO to prepare a detection zone. The ITO glass is taken as a working electrode for modifying an antibody LDL-C, enzyme for enzymatic reaction and an electron transfer medium layer upon layer. A platinum filament electrode is taken as a counter electrode, silver / silver chloride electrode can be taken as a reference electrode, and an electrochemistry work station is taken as signal output equipment. According to the invention, LDL-C and total cholesterol concentration can be detected; preparation of a micro channel detection area can simply the experiment operation and greatly reduce the detection sample amount; a modification liquid employed on the electrode is the green and nontoxic environmental protection material, and the cost is low.

Description

technical field [0001] The invention belongs to the technical field of experimental instruments, and in particular relates to the preparation and application of an immunosensor for electrochemical detection of low-density lipoprotein cholesterol. technical background [0002] Electrochemical immunosensor can directly obtain the composition information of complex systems, has many advantages such as high selectivity, fast transmission speed, easy miniaturization, and can be operated in turbid solution, so it is an ideal analytical tool. In recent years, with the continuous deepening of research on antibodies and nanomaterials, the importance of electrochemical immunosensors in the field of biochemical sensor development and commercialization has gradually become prominent, and can be widely used in clinical testing, food safety, environmental monitoring, medical and health, etc. field. [0003] The high incidence, high disability rate and high mortality rate of cardiovascula...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/36
Inventor 吴琼徐琳黄维
Owner NANJING UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap