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A kind of tissue culture and rapid propagation method of Acer aureus

A technique for rapid propagation of Acer aureus aureus and tissue culture, which is applied in the field of plant tissue culture and can solve problems such as slow start, poor rooting performance, and low multiplication coefficient

Inactive Publication Date: 2017-10-24
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention provides a method for tissue culture and rapid propagation of Acer aureus aureus, which overcomes the deficiencies of slow startup, low multiplication coefficient, poor rooting performance and low survival rate in tissue culture of Acer aureus aureus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] After the dormant buds were pulled out in spring, new shoots of about 5 cm were collected, and the surface was disinfected and inoculated in the medium containing 6-BA (0, 0.5, 1.0 mg / L), 6 g / L agar powder, and 30 g / L sucrose in different concentration gradients. In MS medium, cultured at 25±2°C, 14 hours of light, light intensity 2000lx, to induce germination of axillary buds. The germination time of axillary buds and the state of germinated stem segments were counted. The results showed that the germination time of axillary buds of explants in the medium containing 0mg / L, 0.5mg / L, and 1.0mg / L 6-BA was 25 days, 15 days, and 12 days, respectively, and adding 6-BA could advance the start time of axillary buds , the germinated shoots in the medium containing 0.5-1.0 mg / L 6-BA were in normal condition, the leaves were flat, and the germinated shoots elongated faster.

Embodiment 2

[0022] Collect new shoots of about 5cm after the dormant buds are pulled out in spring, inoculate them in MS medium containing 0.5mg / L 6-BA, 6g / L agar powder, and 30g / L sucrose after surface disinfection, at 25±2℃ , 14 hours of light, culture under the condition of light intensity 2000lx, induce axillary bud germination. The tender stems obtained from the germination of axillary buds were cut into 2-3cm segments with 2 petioles, and inoculated in TDZ containing 0.5mg / L 6BA, 0.05mg / L NAA, and different concentration gradients (0, 0.01, 0.05, 0.1mg / L), 6g / L agar powder, 30g / L sucrose, 0.25g / L MES, 0.2g / L L-glutamine MS medium, cultured at 25±2°C, 14 hours light, light intensity 2000lx Proliferate culture. After 50 days of culture, the multiplication factor was counted. The results showed that the average number of buds per stem segment multiplied in the medium containing 0mg / L, 0.01mg / L, 0.05mg / L, and 0.1mg / L TDZ were 2, 4.8, 5.8, and 2.6, respectively. . The buds prolifera...

Embodiment 3

[0024] Collect new shoots of about 5cm after the dormant buds are pulled out in spring, inoculate them in MS medium containing 0.5mg / L 6-BA, 6g / L agar powder, and 30g / L sucrose after surface disinfection, at 25±2℃ , 14 hours of light, culture under the condition of light intensity 2000lx, induce axillary bud germination. Cut the tender stems obtained from the germination of axillary buds into 2-3cm segments with 2 petioles, and inoculate them in a medium containing 0.5mg / L 6BA, 0.05mg / L NAA, 0.05mg / L TDZ, 6g / L agar powder, 30g / L In the MS medium of L sucrose, 0.25g / L MES, 0.2g / L L-glutamine, at 25±2°C, 14 hours of light, light intensity of 2000lx under the condition of culture for proliferation culture. Inoculate the vigorous growth and 3-4cm long single buds obtained from the proliferation in IBA (0.01, 0.05, 0.1 mg / L) medium with different concentration gradients near 1 / 2 MS, and NAA with different concentration gradients near 1 / 2 MS (0.01, 0.05, 0.1mg / L) medium, carry out ...

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Abstract

The invention discloses an Acer negundo 'Aurea' tissue culture rapid propagation method. The method includes the following steps that newly grown twigs about 5 cm in length are collected after spring dormant buds sprouting, the twigs are inoculated into an MS culture medium into which 6- benzylaminopurine with the concentration ranging from 0.5 mg / L -1.0 mg / L is added after the surfaces of the twigs are sterilized, and axillary buds are induced to sprout; obtained tender stems are clipped into segments ranging from 2 cm to 3 cm in length and inoculated into the MS culture medium, and enrichment culture is conducted; when single buds growing strongly and ranging from 3 cm to 4 cm in length are inoculated into the 1 / 2 MS culture medium, rooting culture is conducted; when the roots grow to 3 cm to 6 cm, a bottle cover is opened, and seedlings are transplanted 3 days after hardening-seedling is conducted. According to the method, the detached material axillary buds of the Acer negundo 'Aurea' can be promoted to sprout quickly by adjusting the culture medium components and controlling the culture conditions, test pipe seedling stem segments are induced to directly generate adventitious buds without a callus, a great number of fibrous roots exist, the transplant survival rate reaches 85%, and an effective way is provided for industrialized seedling production of the Acer negundo 'Aurea'.

Description

technical field [0001] The invention relates to a method for plant tissue culture, in particular to a method for tissue culture and rapid propagation of Acer aurantiaceae. Background technique [0002] Acer negundo 'Aurea' belongs to the Acer family (Aceraceae) and is a cultivar of Acer compound leaf. It is a deciduous tree and is an excellent ornamental tree species introduced from Europe in recent years. The golden-leaved maple leaves grow vigorously, with smooth and golden-yellow twigs; the odd-numbered pinnate compound leaves are opposite, the leaves are larger, and the leaf color is soft, golden-yellow in spring, gradually turning yellow-green, and has a very high ornamental value. The golden-leaf maple has a wide range of growth, strong resistance, drought resistance, cold resistance (tolerance of -40-45 °C low temperature), mild saline-alkali soil resistance, smoke resistance, and strong root germination. Because of its strong fitness and high ornamental value, it is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 贾小明张焕玲石引刚张波贾昕晔陈星星王丽娜曹晴
Owner NORTHWEST A & F UNIV