Manufacturing method of liver depression type gastric ulcer disease combined animal model

An animal model, a technology of gastric ulcer disease, applied in the field of breeding vertebrates, can solve problems such as the inability to reflect the pathogenic relationship of mental factors, and achieve good operability and good reproducibility

Inactive Publication Date: 2015-09-23
LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology

[0005] Animal models of gastric ulcer in modern medical research are mostly simply simulating gastric ulcer disease, which cannot reflect the pathogenic relationship of s

Method used

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  • Manufacturing method of liver depression type gastric ulcer disease combined animal model
  • Manufacturing method of liver depression type gastric ulcer disease combined animal model
  • Manufacturing method of liver depression type gastric ulcer disease combined animal model

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Experimental program
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Effect test

Embodiment 1

[0026] This embodiment provides a method for making an animal model of liver-stagnation type gastric ulcer syndrome combination, which includes the following steps.

[0027] Step 1. Prepare an animal model of liver depression syndrome.

[0028] P.Willner's traditional chronic unpredictable stimulation model was used for 21 days, and the stimulation included: water deprivation for 24 hours; food deprivation for 24 hours; tail clamping for 5 minutes; shaking: 1 time / s, 5 minutes; day and night reversal; electric shock on the soles, voltage 50mV, stimulated once every 5s, with an interval of 5s, and stimulated 10 times in total; restrained for 2h, so that the head of the rat was at the opening end of the behavior restraint cylinder, and it is advisable not to affect its breathing; swim at 4°C for 5min. One stimulus is randomly selected every day, each stimulus is used 3 times on average, and the same stimulus is not repeated on the same individual in two days.

[0029] Step 2, p...

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Abstract

Provided is a manufacturing method of a liver depression type gastric ulcer disease combined animal model. A gastric ulcer model is manufactured through an acidified ethanol solution belly-pouring method on the basis of manufacturing a liver depression model in traditional Chinese medicine syndrome differentiation through P.Willner traditional chronic unpredictability stimuli according to a Chinese medicine basic theory, comparison is conducted through an established animal macroscopic physical sign collection and evaluation table, and the animal model manufactured through the method conforms to clinical human disease attributes; the important attribute, namely, the lack of pleasant sensation, of human depression can be copied to an animal through the chronic unpredictability stimuli, and ulcer symptoms are accordingly exacerbated. In addition, the manufactured animal model better embodies the ordinary state of the gastric ulcer disease progress, clinical similarity is considered during model manufacturing, mechanisms of appearance, histomorphology, relapse and union of gastric ulcer are closest to those of a human body, it is avoided that whether it is pains and sag of operative wound type modeling or after-depression sag can not be distinguished, and convenience is provided for subsequent development.

Description

technical field [0001] The invention relates to the technical field of breeding vertebrates, in particular to a method for making an experimental animal model for the basic research of liver depression type gastric ulcer disease and the normative, standard and scientific evaluation of traditional Chinese medicine. Background technique [0002] Gastric ulcer is a common clinical digestive system disease, which can occur in various ages, including children, young people and the elderly. About 10% of people suffer from this disease at some stage in their lives, and about 1% can be transformed into gastric cancer. Therefore, the active stage of gastric ulcer is listed as a high-risk precancerous state by WHO, which seriously threatens human health. The incidence of gastric ulcer is closely related to smoking, drinking, irregular diet, living habits, work and external pressure, and mental and psychological factors. Clinically, it can be manifested as dull pain in the epigastric ...

Claims

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Application Information

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IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 任路林卉贾连群李丹李春日赵守彰付沈康邓雪孙剑端李静
Owner LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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