Method for inducing Muse cells in adult bone marrow into neural precursor cells (NPCs)

Abstract

The invention relates to the technical field of cytobiology, and provides a method for separating Muse cells from adult bone marrow and inducing into Muse-NPCs in vitro and also performs identification on the Muse cells and induced Muse-NPCs in the aspects of morphologic observation, cell phenotype, multiway differentiation capacity, tumorigenesis and the like. The related molecular markers for the Muse cells and Muse-NPCs cultured by the method are completely expressed. The invention also provides the Muse cells separated from adult bone marrow according to the method and the Muse-NPCs obtained by performing induced differentiation on the Muse cells. The Muse-NPCs are successfully induced to form in vitro, and can provide new favorable seed cells for cellular therapy of nerve injury and tissue engineering therapy in future by utilizing the characteristics of continuous proliferation, multiway differentiation and self derivation.

Description

technical field [0001] The invention belongs to the technical field of cell biology and relates to a method for separating and inducing differentiation of cells, specifically a method for isolating Muse cells from adult bone marrow and inducing neural precursor cells in vitro. Background technique [0002] After spinal cord injury, a large number of neurons and glial cells in the central nervous system undergo apoptosis and death, and the few remaining neural stem cells cannot fully replenish the lost nerve cells, and the regenerative ability of damaged neurons is relatively weak. This is one of the important reasons leading to the impairment of axon regeneration and functional recovery. In order to supplement the lost nerve cells, it is necessary to perform cell therapy on the injured spinal cord, and how to select suitable seed cells is the primary and key problem facing cell therapy. Various stem cells are widely used because of their multi-directional differentiation po...

AI Technical Summary

Problems solved by technology

(3) Induced pluripotent stem cells (iPS cells): pluripotent stem cells that are similar to ES cells in terms of cell doubling ability and differentiation ability can be obtained from somatic cells, and there is no ethical controversy; but add 4 Each method of reprogramming genes or replacing defective genes in diseased cells may have tumorigenic side effects, and there is no clear method to detect and eliminate the undifferentiated tumorigenic cells it contains

[0005] However, there is no report on the application of Muse cells in the nervous system, especially in spinal cord injury, and there is no literature report on isolating Muse cells from adult bone marrow and inducing neural precursor cells (NPCs) in vitro

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