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General medium for determination of lactobacillus resistance to drugs and use thereof

A technology of culture medium and lactic acid bacteria, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve problems such as result deviation and influence, and achieve the effects of easy operation, wide application prospects, and reduced labor intensity

Active Publication Date: 2015-09-30
BEIJING JUNLIKANG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] To sum up, there is currently no culture medium suitable for the determination of drug resistance of three different types of lactic acid bacteria. It is a medium for the better growth of lactic acid bacteria. At the same time, the added ingredients cannot affect various test antibiotics, causing deviations in results

Method used

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  • General medium for determination of lactobacillus resistance to drugs and use thereof
  • General medium for determination of lactobacillus resistance to drugs and use thereof
  • General medium for determination of lactobacillus resistance to drugs and use thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Use the substratum developed by the present invention to cultivate lactic acid bacteria, the specific method is as follows:

[0035] 1) Solid plate preparation: according to the formula, each 1000mL medium contains tryptone 5g, soytone 5g, beef extract powder 5g, yeast powder 3g, glucose 20g, ascorbyl palmitate 1g, pyridoxal hydrochloride 2.6mg, half Cystine Hydrochloride 0.5g, Poloxamer 1881.2g, Magnesium Sulfate 0.2g, MnSO 4 .4H 2O 0.05g, β-glycerophosphate disodium 19g, agar 13g, the balance is water, and the pH is 6.2±0.2 to prepare the culture medium. Weigh each component according to the name and content of each component in the formula, add each component of the above medium into deionized water, stir, heat until completely dissolved, adjust the pH to 6.2±0.2, and after autoclaving, cool to 45°C-55°C, aseptic operation, pour into the plate, so that the thickness of the medium is 4mm-5mm, cool to room temperature, and use it after it is completely solidified;

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Embodiment 2

[0041] The culture medium formula that adopts in the present embodiment is that every 1000mL medium contains tryptone 10g, beef extract powder 5g, yeast powder 3g, glucose 20g, ascorbyl palmitate 1g, pyridoxal hydrochloride 2.6mg, cysteine Hydrochloride 0.5g, poloxamer 1.2g, magnesium sulfate 0.2g, MnSO4.4H2O0.05g, β-glycerophosphate disodium, 19g, agar 13g, balance water, pH 6.2±0.2.

[0042] All the other steps are the same as in Example 1. Microscopic examination results show that Lactobacillus delbrueckii subsp. bulgaricus CICC6047, Lactobacillus plantarum ATCC8014, Bifidobacterium bifidum ATCC11863 and Streptococcus thermophilus ATCC19258 inoculated on the solid plate all have colony growth, illustrating The medium used can be used for the cultivation of Lactobacillus, Bifidobacterium and Streptococcus thermophilus at the same time.

Embodiment 3

[0044] The medium formula adopted in this example is that every 1000mL medium contains 10g of soytone, 5g of beef extract powder, 3g of yeast powder, 20g of glucose, 1g of ascorbyl palmitate, 2.6mg of pyridoxine hydrochloride, and 1.2mg of poloxamer. g, magnesium sulfate 0.2g, MnSO 4 .4H 2 O 0.05g, β-glycerophosphate disodium, 19g, agar 13g, the balance is water, pH is 6.2±0.2.

[0045] All the other steps are the same as in Example 1. Microscopic examination results show that Lactobacillus delbrueckii subsp. bulgaricus CICC6047, Lactobacillus plantarum ATCC8014, Bifidobacterium bifidum ATCC11863 and Streptococcus thermophilus ATCC19258 inoculated on the solid plate all have colony growth, illustrating The medium used can be used for the cultivation of Lactobacillus, Bifidobacterium and Streptococcus thermophilus at the same time.

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Abstract

The invention discloses a general medium for determination of lactobacillus resistance to drugs and a use thereof. Each 1000mL of the general medium comprises 7-15g of peptone, 3-7g of beef extract powder, 2-4g of yeast powder, 15-25g of glucose, 0.8-1.2g of scorbic palmitate, 2.6mg of vitamin B6, 0.5-1g of cysteine hydrochloride, 1.0-2.0g of poloxamer, 0.1-0.3g of magnesium sulfate, 0.05g of MnSO4.4H2O, 15-25g of disodium beta-glycerophosphate, 15-20g of agar and the balance water and has pH of 6.2+ / -0.2. The general medium can be used for simultaneous growth of three different strains in lactobacillus, has components which do not influence various test antibiotics, greatly reduces a detection cost, reduces processes and reduces labor intensity of workers.

Description

technical field [0001] The invention relates to a culture medium used for the determination of bacterial drug resistance, in particular to a general culture medium used for the determination of the drug resistance of lactic acid bacteria. Background technique [0002] Lactic acid bacteria (lactic acid bacteria) is a general term for a class of bacteria that ferment sugar and mainly produce a large amount of lactic acid, mainly Lactobacillus, Bifidobacterium and Streptococcus. Lactic acid bacteria are probiotics, which occupy an increasingly important position in the food and pharmaceutical industries. However, with the widespread use of antibiotics, lactic acid bacteria have different degrees of drug resistance to various types of antibiotics (in healthy humans and health products). Antibiotic resistance analysis of lactic acid bacteria and bifidobacteria, Chinese Journal of Food Science, 2009, the 9th phase of the 9th volume), on the other hand, because the gene cloning and...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
Inventor 赵贵明陈颖赵勇胜姬庆龙王娉杨海荣
Owner BEIJING JUNLIKANG BIOTECHNOLOGY CO LTD
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