Composite biological matrix for glaucoma surgery and preparation method thereof

A biological matrix, surgical technique, applied in the fields of surgery, medical science, coating, etc., to achieve long-term stability to prevent scarring, improve long-term treatment effect, and simple process.

Active Publication Date: 2015-10-07
SHAANXI BOYU REGENERATIVE MEDICINE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Aiming at the problem of scarring after filtration surgery, the invention...

Method used

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  • Composite biological matrix for glaucoma surgery and preparation method thereof
  • Composite biological matrix for glaucoma surgery and preparation method thereof
  • Composite biological matrix for glaucoma surgery and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1, using porcine peritoneum or bovine pericardium and purified collagen as raw materials to prepare composite biomatrix

[0041] Step 1. Preparation of Dense Layer Substrate

[0042] Using pig peritoneum or bovine pericardium as raw materials, decellularization is performed according to the method provided by the Chinese patent application number 201410780482.7. Transfer the pig peritoneum or bovine pericardium that has been pretreated and degreased into a hypertonic solution containing 0.05M NaOH for oscillating treatment for 30 minutes, then transfer it to purified water and hypotonic solution for oscillating treatment for 30 minutes, repeat this 5 times, and then use purified Water was shaken and washed 10 times to remove residual decellularization reagents to obtain decellularized porcine peritoneum or bovine pericardium material.

[0043] Use EDC as the cross-linking agent, prepare a cross-linking solution with a concentration of 0.1% (g / 100ml), and car...

Embodiment 2

[0050] Example 2, using pig small intestine and sodium hyaluronate as raw materials to prepare composite biomatrix

[0051] Step 1. Preparation of Dense Layer Substrate

[0052] Using pig small intestine as raw material, the decellularization process is carried out according to the method provided by the Chinese patent No. ZL201310117569.1. The pig small intestine after removing the mucosal layer and lymph nodes and cleaning it was soaked and disinfected with 3% hydrogen peroxide for 2 hours, the muscular layer and serosa layer were scraped off mechanically, and then the obtained small intestinal submucosa was moved into the alkali-salt mixture with pH>11 and soaked for 60 minutes , washed with purified water until neutral to obtain decellularized small intestinal submucosa material.

[0053] With multifunctional epoxy compound (structural formula , n=2) as the cross-linking agent, prepare a cross-linking solution with a concentration of 5% (g / 100ml), and carry out cross-li...

Embodiment 3

[0060] Example 3, using amniotic membrane and bacterial cellulose as raw materials to prepare composite biomatrix

[0061] Step 1. Preparation of Dense Layer Substrate

[0062] Using amniotic membrane as raw material, decellularization is performed according to the method provided by the Chinese patent application number 201310109216.7. After the amniotic membrane material was washed with purified water, soaked in hydrogen peroxide and disinfected, it was transferred into 3M sodium chloride hypertonic solution and oscillated for 60 minutes, then transferred to purified water and oscillated for 60 minutes.

[0063] Spread the decellularized amniotic membrane material on a glass plate, use genipin as a crosslinking agent, prepare a crosslinking solution with a concentration of 0.75% (g / 100ml), and perform decellularized amniotic membrane at a ratio of 20 ml / g at 25°C The material is cross-linked for 24 hours, and then rinsed three times with 20 times the amount (ml / g) of purifi...

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Abstract

The invention discloses a composite biological matrix for glaucoma surgery and a preparation method thereof. The composite biological matrix has a double-layer composite structure formed by aging and freeze-drying a compact base material layer and a loose layer coated on the compact base material layer, wherein the compact base material layer is a cross-linked denuded amniotic membrane or animal tissue membrane, and the loose layer is a porous membrane prepared from a biological macromolecular material. The composite biological matrix is prepared by multiple times of cross-linking modification, so that the retention time of the composite biological matrix in vivo is significantly prolonged, the effect of stably preventing scarring for a long term is achieved, and the long-term treatment effect after filtering surgery can be effectively improved.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a composite biological matrix for preventing scarring of a filtration channel and a preparation method thereof. The composite biological matrix can be applied to glaucoma surgery. Background technique [0002] Glaucoma is the second irreversible blinding eye disease after cataract in my country, and filtration surgery is still the first choice for glaucoma treatment in my country. However, due to the inherent wound healing mechanism of the human body, often more than 20% of patients after filtration will fail due to scarring of the filtration channel. [0003] In order to solve the problem of postoperative scarring, people try to add anti-scarring drugs such as 5-fluorouracil, mitomycin C, etc. during surgery to improve the postoperative therapeutic effect. The short-term effect of using drugs to inhibit scar formation is very obvious, but complications...

Claims

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Application Information

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IPC IPC(8): A61L31/12A61L31/14A61L31/10
Inventor 刘雪梅
Owner SHAANXI BOYU REGENERATIVE MEDICINE CO LTD
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