Saccharification method of potato raw material and production method of liquid fuel
A manufacturing method and technology of potato residue, which can be applied in the directions of biofuel, fermentation, etc., can solve problems such as rising cost and the like
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Embodiment 1
[0059] (1) Analysis of the basic components of potato starch extraction residue
[0060] A freeze-dried product of potato residues (potato starch extraction residues) collected at the starch factory of Beidahuang Potato Group Co., Ltd., Heilongjiang Province, China was used for the saccharification test described later.
[0061] Therefore, first, the component composition of the potato residues used was analyzed by a conventional method. The results are shown in Table 1.
[0062] [Table 1]
[0063]
[0064] In addition, the lower part of Table 1 shows the monosaccharide composition after the polysaccharide in the said potato residue was completely decomposed. The monosaccharide composition is an actual value obtained by completely decomposing potato residues with sulfuric acid. Glucose is basically produced by decomposing starch and cellulose, so the total amount of starch and cellulose is basically the same as the glucose content in the monosaccharide composition.
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Embodiment 2
[0070] (1) Enzyme production using potato residue
[0071] The cellulase-producing bacterium Acremonium cellulolyticum TN strain (preservation number: FERM BP-11452) was cultured with potato residue as a carbon source to make it produce enzyme. In addition, as a control, cultivation was also performed in which powdered cellulose, which is generally used as a carbon source for cellulase production, was used as a carbon source instead of potato residues.
[0072] The composition of the medium used in the cultivation is as follows: 24 g / L KH 2 PO 4 , 1 g / L Tween 80, 5 g / L (NH 4 ) 2 SO 4 , 1.2 g / L MgSO 4 ・7H 2 O, 0.01 g / L ZnSO 4 ・7H 2 O, 0.01 g / L MnSO 4 ・6H 2 O, 0.01 g / LCuSO 4 7H 2 O, 4 g / L urea, 4.7 g / L potassium tartrate monohydrate, and powdered cellulose (Solka Floc (registered trademark); control) or freeze-dried potato residues (5%, 7%, 10% ).
[0073] The cultivation was carried out at a reaction temperature of 30° C. and a stirring speed of 200 rpm. The pre-...
Embodiment 3
[0083] Instead of the Acremonium cellulolyticum TN strain, the filamentous fungus Penicillium NBRC 101300 strain (NITE) NBRC catalog [NBRC Catalog of Biological Resources, Microorganisms, Microorganism-Related DNA Resources, Human -Related DNAResources, Second Edition (2010)], obtained from NBRC), Trichoderma reesei RUT C-30 strain (theAmerican Type Culture Collection (ATCC) no.56765)) under the same conditions as in Example 2, using Potato residue was cultivated as a carbon source, and the obtained enzyme solution was used to saccharify potato residue. For the saccharified solution, the results of measuring the amount of glucose and galactose are shown in Figure 5 .
[0084] The results showed that all three filamentous fungi produced glucose and galactose. Such as Figure 5 As shown, the amount of glucose was the highest when using the enzyme solution derived from Acremonium cellulolyticum, which is particularly preferable for saccharification. However, for the enzyme s...
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