Method for saccharification of potato starting material and method for producing liquid fuel
A manufacturing method and potato nugget technology, which can be applied to biofuels, fermentation, etc., can solve problems such as rising costs
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Embodiment 1
[0059] (1) Analysis of the basic components of potato starch extraction residue
[0060] The freeze-dried potato residue (potato starch extraction residue) collected in the starch factory of Beidahuang Potato Group Co., Ltd. in Heilongjiang Province, China was obtained and used for the saccharification test described below.
[0061] Therefore, firstly, the composition of the potato residue used was analyzed by conventional methods. The results are shown in Table 1.
[0062] [Table 1]
[0063]
[0064] In addition, the lower part of Table 1 shows the monosaccharide composition after complete decomposition of the polysaccharide in the above-mentioned potato residue. The monosaccharide composition is an actual value obtained by completely decomposing potato residue with sulfuric acid. Glucose is basically produced by the decomposition of starch and cellulose, so the total amount of starch and cellulose is basically the same as the glucose content in the monosaccharide composition.
[00...
Embodiment 2
[0070] (1) Enzyme production using potato residue
[0071] The cellulase-producing bacterium Acremonium cellulolyticum TN strain (preservation number: FERM BP-11452) was cultured with potato residue as a carbon source to produce enzymes. In addition, as a control, a culture in which powdered cellulose, which is generally used as a carbon source for cellulase production, was used as a carbon source instead of potato residue was also performed.
[0072] The composition of the medium used in the culture is as follows: 24 g / L KH 2 PO 4 , 1 g / L Tween 80, 5 g / L (NH 4 ) 2 SO 4 , 1.2 g / L MgSO 4 ?7H 2 O, 0.01 g / L ZnSO 4 ?7H 2 O, 0.01 g / L MnSO 4 ?6H 2 O, 0.01 g / L CuSO 4 7H 2 O, 4 g / L urea, 4.7 g / L potassium tartrate monohydrate, and powdered cellulose as a carbon source (Solka Floc (registered trademark); control) or freeze-dried potato residue (5%, 7%, 10%) ).
[0073] The cultivation was carried out at a reaction temperature of 30°C and a stirring speed of 200 rpm. The pre-culture is perfo...
Embodiment 3
[0083] Instead of the Acremonium cellulolyticum TN strain, the filamentous fungus Penicillium NBRC 101300 strain (NITE)’s NBRC Catalogue of Biological Resources, Microorganisms, Microorganism-Related DNA Resources, Human -Related DNA Resources, Second Edition (2010)], obtained from NBRC), Trichoderma reesei RUT C-30 strain (the American Type Culture Collection (ATCC) no. 56765)) under the same conditions as in Example 2 , Use potato residue as a carbon source for cultivation, and use the resulting enzyme solution to saccharify the potato residue. For the saccharification liquid, the results of measuring the amount of glucose and galactose are shown in Figure 5 .
[0084] The results showed that all three filamentous fungi produced glucose and galactose. Such as Figure 5 As shown, the amount of glucose is the highest when the enzyme solution derived from Acremonium cellulolyticum is used, and it is particularly preferred for saccharification. However, for the enzyme solution ...
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