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Cell level-based ultraviolet radiation resistance evaluation method

An evaluation method, cell-level technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problem that there is no anti-ultraviolet radiation evaluation method

Inactive Publication Date: 2015-10-28
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As far as the applicant knows, there is no method for evaluating the resistance to ultraviolet radiation at the cellular level

Method used

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  • Cell level-based ultraviolet radiation resistance evaluation method
  • Cell level-based ultraviolet radiation resistance evaluation method
  • Cell level-based ultraviolet radiation resistance evaluation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1. Extraction of Aloe Bark

[0042] Select the well-growing Aloe arborescens, pick the leaves, wash, and separate the skin and flesh; weigh 25g of Aloe arborescens skin and mix it with 250mL of acetone solution with a volume fraction of 80%, and use a tissue masher to crush and extract the activity of Aloe arborescens. Composition for 5 minutes, then transfer the solute and dregs to a beaker, and homogenize for 3 minutes with a homogenizer at a speed of 12000r / min. After rotary evaporating most of the solution, dilute to volume with 25mL of alcohol with a volume fraction of 70%, and store at -40°C after aliquoting;

[0043] 2. Anti-ultraviolet radiation evaluation method based on cell level

[0044] (1) After lyophilizing the extract of Aloe arborescens bark 1g / mL, it was redissolved with the medium for subsequent use; the HepG2 cell ATCC company (ATCC HB-8065) ​​in the logarithmic growth phase was digested from the culture bottle, and after centrifugal counting, Form...

Embodiment 2

[0053] 1. Extraction method of Cape aloe vera gel

[0054] Select the well-growing Cape Aloe Vera, pick the leaves, wash, and separate the skin and flesh; weigh 25g Mucape Aloe Vera gel and mix it with 80% acetone solution 250mL, use a tissue masher to crush and extract Cape The active ingredient in the aloe vera gel was mixed for 5 minutes, then the solute and dregs were transferred to a beaker, and homogenized by a homogenizer for 3 minutes at a speed of 12000 r / min. After rotary evaporating most of the solution, dilute to volume with 25mL of alcohol with a volume fraction of 70%, and store at -40°C after aliquoting;

[0055] 2. Anti-ultraviolet radiation evaluation method based on cell level

[0056] (1) After lyophilizing the extract of Aloe arborescens bark 1g / mL, it was redissolved with the medium for subsequent use; the HepG2 cell ATCC company (ATCC HB-8065) ​​in the logarithmic growth phase was digested from the culture bottle, and after centrifugal counting, Formula...

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Abstract

The invention relates to a method for evaluating the performances of phytochemical substances, skin care products and other cosmetics, and concretely relates to a cell level-based ultraviolet radiation resistance evaluation method. The radiation resistance effect of a substance to be measured is assessed without toxicity or anti-proliferative concentration. The substance to be measured has ultraviolet radiation resistance activity or not can be obtained within a short period through adopting the assessment method, and is evaluated through the steps of dyeing cells by a cell reactive dye and converting the light absorption value into the cell survival rate. HepG2 cells have short growth cycle, and the ultraviolet radiation effect rapidly acts on the cells, so the method has the advantages of short experiment cycle and simple operation.

Description

technical field [0001] The invention relates to a method for evaluating the performance of phytochemical substances, skin care products and other cosmetics, in particular to a method for evaluating anti-ultraviolet radiation based on the cell level. Background technique [0002] In daily life, human beings are harmed by ultraviolet radiation all the time. Any heating object can emit ultraviolet rays when the temperature is as high as 1000 ℃ ~ 2000 ℃. According to the different wavelengths of ultraviolet radiation, it is divided into three bands: UVA (320nm-400nm), UVB (275nm-320nm), UVC (230nm-275nm). Depending on the energy and wavelength, ultraviolet rays can cause different degrees of acute or delayed reactions on the human epidermis, generate free radicals and reactive oxygen species (ROS), and cause DNA damage and mutations. UVC is a short-wave ultraviolet light, and its high-energy radiation can directly cause DNA and protein damage and cause cell apoptosis. At the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02
Inventor 郭新波赖沁润刘瑞海扶雄
Owner SOUTH CHINA UNIV OF TECH
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