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A kind of breeding method of triploid hybrid of mustard for roots

A technology for root-use mustard and cultivation method, which is applied in the field of cultivation of root-use mustard triploid hybrids, can solve the problems of inconsistency of groups, disorganized seeds, and inability to mass-produce root-use mustard seeds, etc., and achieves the effect of increasing yield

Inactive Publication Date: 2017-07-07
ANHUI SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If diploid and tetraploid root mustard seedlings are planted in the same field, not only diploid and tetraploid can cross each other, but diploid and tetraploid groups will also pollinate and hybridize each other, so The seeds produced will be messy, including triploid hybrids, tetraploid and diploid seeds, making the population inconsistent and unable to meet the standardization requirements of agricultural production
Due to the large amount of flowers, small fruits, and few seeds per flower, it is obviously impossible to mass-produce root mustard seeds by artificial hybridization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] Steps for cultivating root mustard triploid hybrids:

[0021] (1) Doubling and breeding of root mustard germplasm materials: Sow the root mustard material to be doubled and raise seedlings, and perform double mutagenesis by instilling colchicine solution at the cross stage, and detect and collect four seedlings after they grow up. Ploid plants were isolated and propagated to produce tetraploid root mustard seeds;

[0022] (2) Cultivation of root mustard seedlings: In the tissue culture room, the leaves of diploid and tetraploid root mustard were cultured in tissue culture, and a large number of plants with the same genotype and reproductive characteristics from a single plant were bred. Root mustard seedlings;

[0023] (3) Hybridization of tetraploid root mustard and diploid root mustard: the tetraploid root mustard seedlings and the diploid root mustard seedlings with the same genotype and reproductive characteristics were planted alternately in rows In the same fiel...

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Abstract

The invention discloses a method for cultivating triploid hybrids of Brassica juncea for roots, first performing chromosome doubling on part of the germplasm of Brassica juncea for roots, and then performing tissue culture on tetraploid and diploid roots with Brassica brassica strains, A large number of tetraploid and diploid root mustard seedlings with the same genotype and reproductive characteristics were amplified respectively, and the above two seedlings were planted in the same field alternately in rows for hybrid seed production. Self-fertilization of mustard is not fruitful, and triploid hybrids of root mustard are obtained through natural hybridization. According to the hugeness of triploid plant organs, the fleshy root yield of triploid root mustard will be greatly improved.

Description

technical field [0001] A method for cultivating triploid hybrids of root mustard, relating to the technology of root mustard triploid breeding, more specifically a method of hybridizing diploid root mustard and tetraploid root mustard for triple breeding Method for body roots using mustard hybrids. Background technique [0002] Root mustard is a vegetable variety for pickling, and its pickled products, such as root mustard shreds and root mustard slices, have become more and more popular in the market in recent years. At present, the production of root mustard adopts conventional varieties or even farm varieties from various places. The yield is low and unstable, and the quality is uneven. Root mustard is a vegetative vegetable, and the use of triploid breeding can greatly increase its economic output, and at the same time bring new economic growth points to the seed industry. [0003] Among the crops, many crops have bred triploid high-yield and high-quality new varieties...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01H1/02
Inventor 王治华程亮林平
Owner ANHUI SCI & TECH UNIV