Method for preparing cigar tobacco leaves by virtue of oxidase fermentation

A cigar tobacco leaf and oxidase technology, which is applied in the directions of tobacco, application and treatment of tobacco, can solve the problems of less research and application of tobacco leaves, and achieve the effects of improved flammability, improved gray score, and improved bitterness.

Active Publication Date: 2015-11-11
HUBEI CHINA TOBACCO IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, there are few studies and applications of biological enzymes on the quality of tobacco leaves in the tobacco industry.

Method used

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  • Method for preparing cigar tobacco leaves by virtue of oxidase fermentation
  • Method for preparing cigar tobacco leaves by virtue of oxidase fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The method for preparing cigar tobacco leaves by mixed enzyme fermentation comprises the following steps:

[0030] 1) Alcoholize 30kg of the upper part of the cigar filler under natural conditions for one year, and then remoisturize the upper part under vacuum;

[0031] 2) dissolving the mixed enzyme in deionized water to obtain a mixed enzyme aqueous solution, wherein the polyphenol oxidase enzyme activity in the oxidase aqueous solution is 18 U / ml, and the peroxidase enzyme activity is 4.8 U / ml;

[0032] 3) Put the leaf petiole end of the tobacco leaves treated with moisture regain in step 1) in the mixed enzyme aqueous solution to soak the handle for 30 minutes, and then spray the mixed enzyme aqueous solution on the tobacco leaves according to the weight ratio of 1:10 to obtain the tobacco leaves containing the mixed enzyme, wherein, per gram In the tobacco leaves containing mixed enzymes, the enzyme activity of polyphenol oxidase is 1.8U / ml, and the activity of per...

Embodiment 2

[0036] The method for preparing cigar tobacco leaves by peroxidase fermentation comprises the following steps:

[0037] 1) Aging 30kg of the middle part of the cigar filler under natural conditions for one year, and then re-moisture the upper part of the smoke under vacuum conditions;

[0038] 2) dissolving the peroxidase in deionized water to obtain a peroxidase aqueous solution, wherein the peroxidase enzyme activity in the peroxidase aqueous solution is 6 U / ml;

[0039] 3) Place the tobacco leaf petiole end of step 1) remoisturized in the peroxidase aqueous solution to soak the handle for 20 minutes, then spray the peroxidase aqueous solution on the tobacco leaves in a weight ratio of 1:10 to obtain peroxidase-containing Tobacco leaves, wherein, per gram of tobacco leaves containing peroxidase, the peroxidase enzyme activity is 0.6U; let stand for 24 hours to balance the water in the tobacco leaves, and then adjust the moisture content of the tobacco leaves to 24%;

[0040...

Embodiment 3

[0043] The method for preparing cigar tobacco leaves by polyphenol oxidase fermentation comprises the following steps:

[0044] 1) Aging 30kg of the lower part of the cigar filler under natural conditions for one year, and then re-moisture the upper part of the smoke under vacuum conditions;

[0045] 2) dissolving polyphenol oxidase in deionized water to obtain an aqueous solution of polyphenol oxidase, wherein the activity of polyphenol oxidase in the aqueous solution of polyphenol oxidase is 15 U / ml;

[0046] 3) Put the petiole end of the tobacco leaves treated in step 1) in the polyphenol oxidase aqueous solution to soak the stalk for 10 minutes, and then spray the polyphenol oxidase aqueous solution on the tobacco leaves according to the weight ratio of 1:10 to obtain polyphenol oxidase containing polyphenol oxidase. Tobacco leaves, wherein, per gram of tobacco leaves containing polyphenol oxidase, the enzyme activity of polyphenol oxidase is 1.5U; standing for 24 hours to...

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Abstract

The invention discloses a method for preparing cigar tobacco leaves by virtue of oxidase fermentation. The method comprises the following steps: immersing the leaf stalk ends of moisture-regained tobacco leaves into an aqueous oxidase solution for 10-30 minutes, and then spraying the aqueous oxidase solution onto the tobacco leaves in the weight ratio of 1: (5-15) to obtain the tobacco leaves containing the oxidase; standing to keep moisture balance in the tobacco leaves, and then fermenting the tobacco leaves having the moisture content within the range of 25+/-1% under the conditions of a temperature ranging from 35 to 45 DEG C and the humidity of 65-75% for 10-60 days, thereby obtaining the cigar tobacco leaves. According to the method, the cigar tobacco leaves are taken as the object of study, the bitter taste of the cigars obtained by virtue of fermentation is improved obviously, and the cigars are all improved in aroma characteristic, smoke characteristic, taste characteristic, flammability and gray score; the quality of the cigars is improved.

Description

technical field [0001] The invention relates to the field of cigar processing, in particular to a method for preparing cigar leaves by fermenting oxidase. Background technique [0002] Tobacco is a special consumer product, and smoking style is the main factor whether it can be accepted by consumers. In recent years, in order to create cigar products that Chinese people smoke, the product style of "Chinese cigars" has emerged as the times require, and its unique style characteristics have gradually been accepted by consumers. [0003] Chinese-style cigars are domestic cigars with unique aroma style and taste characteristics, which are suitable for the needs of Chinese consumers. Different from European and American cigars, Chinese-style cigars are mainly made of tobacco leaves with special taste grown in China's special geographical and climatic conditions, using traditional Chinese cigar technology, and developed according to the needs of Chinese consumers and the needs of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A24B15/20
Inventor 胡捷张宁罗公金刘利平周斌
Owner HUBEI CHINA TOBACCO IND
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