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A kind of black beetle attractant composition and its application

A technology of dark gill beetle and composition, applied in the directions of application, attractant, biocide, etc., can solve the problems of difficulty in popularization, high replacement cost, low sustainability, etc., and achieves low price, strong autonomy, and cost reduction. Effect

Inactive Publication Date: 2017-11-17
PHEROBIO TECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main ingredients are L-isoleucine methyl ester and linalool. The trapping effect is good, but due to the low sustainability, it causes high replacement costs and is difficult to popularize.

Method used

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  • A kind of black beetle attractant composition and its application
  • A kind of black beetle attractant composition and its application
  • A kind of black beetle attractant composition and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The Y-type olfactometer of embodiment 1 single-component synergist is measured

[0032] In a room with clean air at 25°C, a relative humidity of about 70%, use a 3cm*20cm Y-shaped olfactometer, and according to the characteristics of nocturnal sexual activities of scarab beetles, conduct biometric measurements at 20:00-23:00, using red Light source observation measurement results. Take 20 μl of the single-component compound of the synergist and drop it on a filter paper strip, and place it in one side arm of a Y-shaped olfactometer, and put an equal amount of n-hexane in the other side arm as a control. Insert a dark gill beetle fasting for 24 hours at a distance of 3 cm from the main arm of the Y-shaped olfactory instrument. If the beetle stays in any side arm for more than 1 min, it is considered to have made a choice; if it stays at the main arm for 10 min, then Considered unresponsive. Repeat for 30 dark gill beetles, 15 males and 15 males. The t-test was perform...

Embodiment 2

[0039] Example 2 Determination of the antennal potential activity of the black beetle to 6-methyl-5-hepten-2-one

[0040] Prepare 6-methyl-5-hepten-2-one into 0.01, 0.1, 1, 10, 100 μg / μL n-hexane solutions for use. Use tweezers to carefully remove the antennae of the black beetle, stick the antennae on the conductive rod with conductive glue, and keep the gills of the antennae open. The mouth of the air supply tube is perpendicular to the antennae, and the distance from the antennae is about 1cm. Take 10 μL of the solution and drop it on a 2cm*0.5cm filter paper sheet. reaction on the instrument. Before the treatment, do a control with the same amount of n-hexane. The test concentration ranged from low to high, and 6 antennae (3 male and 3 antennae) were used for each test concentration, and the test was repeated 3 times for each antennae. During the test, the stimulation time was 0.5s, the interval between two times was not less than 1min, and the carrier gas flow rate was...

Embodiment 3

[0042] Example 3 Determination of the antennal potential activity of the black beetle to 2-ethyl-1-hexanol

[0043] Prepare 2-ethyl-1-hexanol into 0.01, 0.1, 1, 10, 100 μg / μL n-hexane solutions for use. Use tweezers to carefully remove the antennae of the black beetle, stick the antennae on the conductive rod with conductive glue, and keep the gills of the antennae open. The mouth of the air supply tube is perpendicular to the antennae, and the distance from the antennae is about 1cm. Take 10 μL of the solution and drop it on a 2cm*0.5cm filter paper sheet. reaction on the instrument. Before the treatment, do a control with the same amount of n-hexane. The test concentration ranged from low to high, and 6 antennae (3 male and 3 antennae) were used for each test concentration, and the test was repeated 3 times for each antennae. During the test, the stimulation time was 0.5s, the interval between two times was not less than 1min, and the carrier gas flow rate was 20mL / min. ...

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Abstract

The invention discloses a black beetle lure composition and an application thereof, which belong to the technical field of preparation of insect sex pheromones. The diabolica attractant composition comprises diablo sex pheromone and a synergist, and the weight ratio of the diablo sex pheromone to the synergist is 1: (0.5-100), wherein the diablo The main components of sex pheromone are isoleucine methyl ester and R-(-)-linalool, and the synergist is selected from 6-methyl-5-hepten-2-one, 2-ethyl-1 - at least one of hexanol, octanal and nonanal. The black beetle attractant composition provided by the invention can be accurately applied to the detection and report of the black beetle insect situation, a large amount of trapping, and interfering with mating, and is of great significance for the comprehensive control of the black beetle.

Description

technical field [0001] The invention relates to the technical field of preparation of insect sex pheromones, in particular to a black beetle attractant composition and application thereof. Background technique [0002] Black gill beetle (Holotrichia parallela), also known as Lachnostema morose Waterhouse, belongs to Coleoptera (Coleoptera), scarabidae (Scarabeidae), is an important worldwide underground pest, especially in China, India and Japan. Both adults and larvae of the black beetle can cause serious damage. Adults feed on the leaves of trees and shrubs such as elms, poplars, pears, and apples, and have the characteristics of overeating on fixed trees and sections of fixed trees. They also eat leaves of crops such as peanuts, soybeans, corn, and sunflowers. The larvae mainly damage field crops such as peanuts, soybeans, sweet potatoes, and wheat, causing heavy losses, and in severe cases, even crop failure. [0003] The dark gill beetle is one of the three dominant s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N37/44A01P19/00A01N31/02A01N35/02
Inventor 李克斌尹姣梅向东张帅曹雅忠费仁雷刘丹丹张开心衣建坤孙庆胜
Owner PHEROBIO TECHNOLOGY CO LTD
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