Method for excising immunogenicity terminal peptide through pepsin

Abstract

The invention discloses a method for excising immunogenicity terminal peptide through pepsin, and belongs to the technical field of peptide treatment. According to the method for excising the immunogenicity terminal peptide through the pepsin, in suspension liquid manufactured by using cowhide as a raw material, terminal amidogen and carboxyl are excised through the pepsin. The method includes the steps that the pepsin is dissolved in acetic acid of 0.5 M to be made into a pepsin solution of 10 mg/ml, then the pepsin solution is transferred into tissue homogenate till the concentration is 0.1 mg/ml, namely 1 ml of the pepsin solution is added into every 100 ml of the homogenate, the mixture is evenly mixed, and digestion is conducted for 7-10 days at the indoor temperature. The method is simple in technology and easy to operate. Through the process, a spherical area containing bovine collagen molecule amidogen and carboxyl terminal (terminal peptide) in the cowhide tissue suspension liquid can be effectively excised, the natural spatial helical segment structure and biological activity of collagen are maintained, and therefore the potential immunogenicity of the bovine collagen is removed.

Description

technical field [0001] The invention belongs to the technical field of peptide treatment. Background technique [0002] Cowhide is often used to make collagen. Generally, cowhide needs to be processed into a uniform suspension before the production of collagen. The existing conventional production of collagen will directly proceed to the next step. However, because the suspension of cowhide after treatment becomes The presence of amino and carboxyl groups at the end of the natural triple helix is ​​immunogenic, so the quality of collagen is affected during the production of bovine collagen. Contents of the invention [0003] The object of the present invention is to provide a method for removing immunogenic telopeptides by pepsin removing terminal amino and carboxyl groups from a suspension prepared from cowhide. [0004] In the present invention, pepsin is dissolved in 0.5M acetic acid to make 10mg / ml pepsin solution, and then transferred to tissue homogenate to 0.1mg / ml...

AI Technical Summary

Problems solved by technology

[0002] Cowhide is often used to make collagen. Generally, cowhide needs to be processed into a uniform suspension before the production of collagen. The existing conventional production of collagen will directly proceed to the next step. However, because the suspension of cowhide after treatment becomes Natural triple helix form, the presence of amino and carboxyl groups at the end is immunogenic, so the quality of collagen is affected during the production of bovine collagen