Stingless blackberry hybrid germplasm breeding and molecular assistant identifying method

Abstract

The invention belongs to the field of plant hybrid breeding, and relates to a stingless blackberry hybrid germplasm breeding and molecular assistant identifying method. The method comprises the following steps: emasculation and bagging, pollen collection, artificial supplementary pollination, pollination post-management, fruit harvest, seed harvest and storage, seed sand-storage and sowing, field management after sowing, superior plant molecular marker identification, superior plant upbringing, evaluation and screening, and the like. According to the method, according to hybrid of stingless and spinose blackberry varieties, and the obtained hybrid seeds are subjected to sowing, seedling cultivation and molecular marker assistant identification, so as to obtain the stingless new hybrid variety with the excellent characteristics of the parents. The background of the hybrid posterity obtained through the method is real and clear, and one effective hybrid method is provided for breeding different types of new blackberry varieties suitable for being produced. The establishment of the breeding method for high-grade stingless blackberry hybrid new germplasm identification by utilizing molecular marker assistance has important significances on breeding of the blackberry new variety with the independent intelligent property right of China.

Description

technical field [0001] The invention belongs to the technical field of plant cross-breeding, and in particular relates to cross-breeding among blackberry varieties and a molecular marker-assisted identification method thereof. Background technique [0002] Blackberry (Blackberry) belongs to the genus Rubus (Rosaceae) Rubus L.) is a characteristic berry fruit tree, whose fruit is rich in vitamin E, anthocyanins and phenolic substances, and has become a special economic forest tree species with great development potential. my country contains rich Rubus plant resources represented by blackberry and raspberry, but due to the late start of research on its fruit, there are very few new varieties suitable for production. [0003] At present, the main cultivars of blackberries in China are basically imported from European and American countries. Most of these varieties are obtained through hybridization methods. Some varieties with excellent traits have thorns after being introduc...

AI Technical Summary

Problems solved by technology

Among the introduced blackberry varieties with good performance in Jiangsu, the Kiowa variety has the characteristics of the largest growth, the heaviest single fruit and the highest yield. The only disadvantage is that the branches and vines have thorns. It is expected to breed new high-quality thornless blackberry hybrid varieties with large growth, high fruit weight and high yield.

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