Method for detecting influence of targeted drug with multiple target points on melanoma cells

A melanoma cell and detection method technology, which is applied in the field of detection of the impact of targeted drugs on the survival and metastasis of melanoma cells, and can solve the problems that single-target inhibitors cannot meet the needs of tumor treatment and melanoma curative effect is weak

Inactive Publication Date: 2015-11-25
HUZHOU CENT HOSPITAL
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Eisen et al. [18] conducted a phase II clinical trial on 37 patients with unresectable melanoma and distant metastases and found that sorafenib monotherapy had little effect on melanoma.
[0006] At present, targeted therapy has become one

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting influence of targeted drug with multiple target points on melanoma cells
  • Method for detecting influence of targeted drug with multiple target points on melanoma cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] Below, the technical solution of the present invention will be described in detail through specific examples.

[0017] 1. Effects of drug treatment on the proliferation inhibition rate, cell cycle and apoptosis rate of melanoma cell A375 cells

[0018] ①Different tumor cells in logarithmic growth phase were digested with 0.25% trypsin to make single cell suspension.

[0019] ② Adjust the cell concentration (1000-10000 cells per well, determined according to the actual growth of different cells) and inoculate in a 96-well culture plate, 0.2ml per well.

[0020] ③Cultivate at 37°C and 5% CO2 for 24 hours. After the growth reaches 70-80%, remove the original culture solution, prepare the treatment drug with DMSO (dimethyl sulfoxide) to form a mother solution, and then stir with the corresponding cell culture solution Diluted to 5 μmol / L, 10 μmol / L, 20 μmol / L, 40 μmol / L, respectively added to 96-well plate, set blank control group, cell control group, sorafenib group, conv...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for detecting influence of a targeted drug with multiple target points on survival and metastasis capacities of melanoma cells. The detection method comprises the following steps: treatment of melanoma cells; RT-PCR detection of expression of a cell metastasis-associated gene PERK; western blot detection of expression of a cell metastasis-associated protein PERK; observation of invasion and metastasis capacity of each group of melanoma cells through Transwell experiments; and integration of data and analysis of effects of the targeted drug with multiple target points on survival and metastasis capacities of melanoma cells. The detection method can systematically detect influence of the targeted drug on survival and metastasis capacities of melanoma cells.

Description

technical field [0001] The invention relates to the technical field of medical detection methods, in particular to a detection method for detecting the influence of multi-target targeted drugs on the viability and metastasis of melanoma cells. Background technique [0002] Melanoma is a malignant tumor originating from melanocytes in the skin, mucous membranes, eyes, and pigmented areas of the central nervous system. It is the skin cancer with the highest mortality rate. It is estimated that in 2009, there were 68,720 melanoma cases in the United States, accounting for 92.1% of skin cancer cases, and 8,650 deaths, accounting for 74.6% of skin cancer deaths, and the annual prevalence rate is rising rapidly. The cause of melanoma is still unclear, and the treatment options are mainly surgery and radiation therapy, supplemented by drug therapy, but the cure rate for middle and advanced patients is very low. The reason is that advanced tumors are prone to metastasis. Tumor met...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/15
Inventor 陆群英吴原戴利成
Owner HUZHOU CENT HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap