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Epitode amino acid sequence of TFG-beta1 (transforming growth factor beta-1) as breast cancer detecting marker and application of epitode amino acid sequence

A TGF-, antigenic epitope technology, applied in the biological field, can solve the problems of poor specificity and low sensitivity, and achieve the effect of high specificity and high sensitivity

Active Publication Date: 2015-12-02
BEIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently reported autoantibody detection methods have low sensitivity and poor specificity, and the false negative rate can be as high as 50%.

Method used

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  • Epitode amino acid sequence of TFG-beta1 (transforming growth factor beta-1) as breast cancer detecting marker and application of epitode amino acid sequence
  • Epitode amino acid sequence of TFG-beta1 (transforming growth factor beta-1) as breast cancer detecting marker and application of epitode amino acid sequence
  • Epitode amino acid sequence of TFG-beta1 (transforming growth factor beta-1) as breast cancer detecting marker and application of epitode amino acid sequence

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Experimental program
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Effect test

Embodiment 1

[0026] kit preparation

[0027] 1 Reagents See Tab.2-7 for reagent preparation.

[0028]

[0029]

[0030]

[0031]

[0032]

[0033]

[0034] 2 operations

[0035] (1) Coating: The enzyme plate should be washed 3 times with washing buffer, the working antigen should be diluted to the working concentration with the coating solution, coated on the enzyme plate, and kept overnight at 4°C.

[0036] (2) Add glutamic acid: Wash 3 times with washing buffer, dilute glutamic acid with coating solution to a concentration of 100 μg / ml, 200 μl per well, incubate at 37°C or room temperature for 1 hour;

[0037](3) Add plasma and quality control control (primary antibody): Wash the enzyme-labeled plate three times with washing buffer, and dilute the plasma to an appropriate concentration with coating solution, generally 1:100-1:500, 100 μl per well, Incubate at 37°C or room temperature for 1 hour;

[0038] (4) Secondary antibody incubation: Wash 3 times with washing ...

Embodiment 2

[0042] Detection of TGF-β1 Auto IgG Antibody in Patients with Breast Cancer

[0043] 1 Sample collection: In this study, 232 breast cancer samples confirmed by radiological examination and histological examination were selected from the Second Hospital of Jilin University and Provincial Cancer Hospital. All serum samples had not undergone any anti-cancer treatment before collection, and had comprehensive clinical data and information. At the same time, 210 healthy control samples were recruited. Clinical interviews and imaging examinations ruled out the possibility of breast cancer. The healthy group and the breast cancer group were matched in gender and age and were comparable ( P >0.05)

[0044] 2 Test results: TGF-β1 autoantibody expression level (Tab.8): There is a statistical difference between the breast cancer group and the healthy control group ( t =-4.231, P <0.001).

[0045] ROC curve analysis: the area under the ROC curve for TGF-β1 auto-IgG antibody detection...

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Abstract

The invention discloses an epitode amino acid sequence of TFG-beta1 (transforming growth factor beta-1) as a breast cancer detecting marker and application of the epitode amino acid sequence, belongs to the technical field of immunology, and provides the antigen amino acid sequence of a breast cancer-related gene TFG-beta1. The TFG-beta1 polypeptide antigen can be applied to detection of the corresponding specific autoantibody in the blood of a breast cancer patient, the autoantibody can be used as the breast cancer marker for evaluating the degree of risk of breast cancer occurrence, and the antigenic polypeptide and the antibody thereof can be used for preparing a reagent for early diagnosis of breast cancer and developing a target medicine for treating breast cancer.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a TGF-β1 polypeptide fragment with a marker for detecting breast cancer. Background technique [0002] Early and accurate diagnosis of breast cancer is the key to prolonging the survival of patients. At present, there is no single effective diagnostic method, and the diagnosis is mainly based on a series of clinical testing items. Breast cancer is a malignant tumor that often occurs in the glandular epithelial tissue of the breast. Its incidence rate ranks first among female malignant tumors, and there is a gradual upward trend. Its malignancy is high, its onset is hidden, and it is easy to spread and transfer. It is more common in the middle and late stages when it is first diagnosed. Therefore, early detection, early diagnosis and early treatment are extremely important for the rehabilitation of breast cancer patients. A large number of studies have shown that tumor-a...

Claims

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Application Information

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IPC IPC(8): C07K14/495G01N33/68G01N33/574
CPCG01N33/57415G01N33/68C07K14/495G01N2333/495
Inventor 李尊严孙世龙
Owner BEIHUA UNIV
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