Separation and extraction and structure identification of novel quorum sensing inhibitor and application thereof
A functional and typical technology, applied in the direction of organic active ingredients, microbial-based methods, and resistance to vector-borne diseases, etc., can solve problems such as no drugs yet, achieve obvious inhibition of quorum sensing, reduce swarm movement, and maintain fermentation conditions. control effect
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Embodiment 1
[0015] Example 1 Fermentative production and separation and purification of compound ring (L-tryptophan-L-serine).
[0016] 1. Fermentation production: marine sludge source water Rhineheimeria QSI02 ( Rheinheimera aquimaris Fermentation culture of QSI02): According to the conventional method of cultivating microorganisms, take an appropriate amount of Rhineheimeria QSI02 (preservation number: CCTCCNO: M2015245) and inoculate it into LB liquid medium (peptone 10g / L; yeast extract 5g / L; NaCl 10g / L, deionized water 1L, 121°C, sterilized for 20 minutes), shake culture for 12h, temperature 28°C, speed 160rmp.
[0017] Take the Rhineheimeria water QSI02 cultured for 12 hours and inoculate it into 2.5L seawater medium (peptone 5g / L; yeast extract 2g / L; ferric phosphate 0.1g / L, seawater filtered by a 0.22μm microporous membrane) 1L, 121°C, sterilized for 20 minutes) in a 5L Erlenmeyer flask with an inoculum size of 4%, and cultured with shaking for 24 hours at a temperature of 28°C a...
Embodiment 2
[0026] Activity test of compound ring (L-tryptophan-L-serine) in Example 2.
[0027] 1. Compound ring (L-tryptophan-L-serine) inhibits quorum sensing activity of Violet bacteria.
[0028] 1.1 Experimental samples and experimental methods: The medium used for activity detection is LB medium, and 1.5% agar is added to the solid medium. The metabolite purple pigment of Violaceum violaceum is strictly regulated by the quorum sensing system (Microbiology, 1997, 143(12): 3703-3711). Violaceum violaceum CV026 is a signal molecule deletion strain, and only when exogenous signal molecule hexanoyl is added homoserine lactone C 6 After -HSL, the production of purple pigment regulated by quorum sensing can be initiated. When interfered by quorum sensing inhibitors, the production of purple pigment can be inhibited, showing a cloudy but opaque halo on the agar plate.
[0029] Violet bacteria plate screening method: Take 20ml of hot-thawed LB soft agar medium, cool it to about 30°C, add 2...
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