Separation and extraction and structure identification of novel quorum sensing inhibitor and application thereof

A functional and typical technology, applied in the direction of organic active ingredients, microbial-based methods, and resistance to vector-borne diseases, etc., can solve problems such as no drugs yet, achieve obvious inhibition of quorum sensing, reduce swarm movement, and maintain fermentation conditions. control effect

Active Publication Date: 2015-12-09
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The study found that the obtained compound ring (L-tryptophan-L-serine) has quorum sensing inhibitory activity against Violet bacteria and Pseudomonas aeruginosa w...

Method used

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  • Separation and extraction and structure identification of novel quorum sensing inhibitor and application thereof
  • Separation and extraction and structure identification of novel quorum sensing inhibitor and application thereof
  • Separation and extraction and structure identification of novel quorum sensing inhibitor and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Fermentative production and separation and purification of compound ring (L-tryptophan-L-serine).

[0016] 1. Fermentation production: marine sludge source water Rhineheimeria QSI02 ( Rheinheimera aquimaris Fermentation culture of QSI02): According to the conventional method of cultivating microorganisms, take an appropriate amount of Rhineheimeria QSI02 (preservation number: CCTCCNO: M2015245) and inoculate it into LB liquid medium (peptone 10g / L; yeast extract 5g / L; NaCl 10g / L, deionized water 1L, 121°C, sterilized for 20 minutes), shake culture for 12h, temperature 28°C, speed 160rmp.

[0017] Take the Rhineheimeria water QSI02 cultured for 12 hours and inoculate it into 2.5L seawater medium (peptone 5g / L; yeast extract 2g / L; ferric phosphate 0.1g / L, seawater filtered by a 0.22μm microporous membrane) 1L, 121°C, sterilized for 20 minutes) in a 5L Erlenmeyer flask with an inoculum size of 4%, and cultured with shaking for 24 hours at a temperature of 28°C a...

Embodiment 2

[0026] Activity test of compound ring (L-tryptophan-L-serine) in Example 2.

[0027] 1. Compound ring (L-tryptophan-L-serine) inhibits quorum sensing activity of Violet bacteria.

[0028] 1.1 Experimental samples and experimental methods: The medium used for activity detection is LB medium, and 1.5% agar is added to the solid medium. The metabolite purple pigment of Violaceum violaceum is strictly regulated by the quorum sensing system (Microbiology, 1997, 143(12): 3703-3711). Violaceum violaceum CV026 is a signal molecule deletion strain, and only when exogenous signal molecule hexanoyl is added homoserine lactone C 6 After -HSL, the production of purple pigment regulated by quorum sensing can be initiated. When interfered by quorum sensing inhibitors, the production of purple pigment can be inhibited, showing a cloudy but opaque halo on the agar plate.

[0029] Violet bacteria plate screening method: Take 20ml of hot-thawed LB soft agar medium, cool it to about 30°C, add 2...

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Abstract

Separation and extraction and structure identification of a novel quorum sensing inhibitor and an application thereof. The invention relates to a compound which is separated and extracted from rheinheimera aquimaris QSI02 and has a quorum sensing quenching activity and an application of the compound. In the invention, the rheinheimera aquimaris QSI02, which is sourced from marine sludge, is subjected to fermentation and cultivation and separation and purification to obtain a cyclodepeptide monomer compound which has a quorum sensing system inhibiting activity of chromobacterium violaceum and pseudomonas aeruginosa, wherein the chemical name of the compound is cyclo(L-tryptophan-L-serine). Compared with the action mechanism of a traditional anti-bacterial medicine, the action mechanism of active compound is that the compound acts on a novel anti-bacterial target point, wherein the compound only interferes in a pathogenic behavior regulated by bacteria quorum sensing without influences on growth of bacteria, so that no bacterial drug resistance is generated theoretically. The compound and derivatives thereof can be used for guiding development of a novel anti-bacterial drug as a guide compound in the fields of clinical researching, agricultural production and the like, so that the compound has important significance on human health and agriculture development.

Description

technical field [0001] The invention belongs to the field of microbial pharmacy, and in particular relates to a kind of Rheinheimeria water QSI02 ( Rheinheimera aquimaris QSI02) fermentation culture, isolation, purification, structure identification and application of compounds with bacterial quorum sensing inhibitory activity. Background technique [0002] Traditional antibiotics target the important life processes of bacteria such as cell wall synthesis, protein synthesis, folic acid synthesis, and DNA supercoiling, and directly kill or inhibit the growth of microorganisms to achieve the purpose of anti-infection. Under stress, bacteria can easily develop drug resistance by changing their own mechanisms. Addressing bacterial resistance has become one of the major health challenges facing mankind. In recent years, studies have found that the bacterial quorum sensing (quorum sensing, QS) system enables bacteria, which are single-celled organisms, to have some functions sim...

Claims

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Application Information

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IPC IPC(8): C07D403/06A61K31/496A61P31/04C12P17/16C12R1/01
CPCA61K31/496C07D403/06C12P17/165Y02A50/30
Inventor 朱虎孙世伟李慧孙郊刘奥周万龙
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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