Flower-shaped silver nanometer particle fluorescence-enhanced substrate and preparation method thereof

Abstract

The invention discloses a flower-shaped silver nanometer particle fluorescence enhanced substrate and a preparation method thereof. The flower-shaped silver nanometer particle fluorescence enhanced substrate is provided with a flower-shaped silver nanometer particle layer which is a sub-monolayer structure composed of a plurality of flower-shaped silver nanometer particles with coverage of 40-80%, wherein the flower-shaped silver nanometer particles have grain sizes ranging from 0.5 to 3 [mu]m and surface nano-grade roughness of 5-300 nm. A silver nitrate solution, polyvinylpyrrolidone and ascorbic acid are subjected to reaction, then to repeated supersonic dispersion and centrifugation to get a flower-shaped silver nanometer particle ethanol dispersion liquid to prepare the substrate. Nano-grade rough structures on the surfaces of the flower-shaped silver nanometer particles amplify fluorescence signals emitted by molecules to 10-100 times. The technology is simple and convenient compared with the prior art without need of extra operation for amplification of fluorescence signals.

Description

technical field [0001] The invention relates to fluorescence analysis technology, more specifically, it relates to a flower-shaped silver nano particle fluorescence enhancement substrate and a preparation method thereof. Background technique [0002] Fluorescence analysis is an important detection method in the biological and medical fields, and the main technical means is ELISA. It uses biological specific recognition of biological molecules such as antigens, antibodies, etc. to detect specific molecules, and then diagnose the disease. However, this technique has certain limitations in the detection of trace biomolecules. Usually in the early stage of the disease, there are few molecules such as antibodies, and the required detection time usually takes days or even weeks, which greatly delays the diagnosis of the disease. Untimely diagnosis usually leads to lower treatment effect and less available treatment methods. This situation exists widely in the diagnosis of type 1...

AI Technical Summary

Problems solved by technology

But on the other hand, the cost of micro-processing technology is huge, requiring clean rooms and related processing equipment

[0006] The other substrate is to spread chemically synthesized nanoparticles on a flat substrate. This substrate is characterized by high signal enhancement, but the signal deviation of each acquisition is large and the reproducibility is low.

In terms of operation, since the size of commonly used nanoparticles is tens to hundreds of nanometers, it is usually necessary to allow aggregation between particles to generate enhanced signals, and the aggregation process is often difficult to control and it is difficult to achieve optimal conditions.

The commonly used solution is temporal particle modification or growth on the substrate surface, but the substrate signal enhancement obtained in this way is limited

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