Method for selecting material collecting period for cold tolerance authentication in momordica charantia L. seedling period
A technology of material collection period and cold resistance, which is applied in the field of selection of cold tolerance identification of bitter gourd seedling stage, can solve the problems of reducing the accuracy and reliability of identification
Inactive Publication Date: 2015-12-16
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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Problems solved by technology
If the physiological and biochemical indicators at this time are used to identify and evaluate the cold tolerance of resources, the accuracy and reliability of the identification will be reduced
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[0030] 1. Test materials
[0031] Select the identified cold-resistant material MC108, intermediate material MC45 and cold-sensitive material MC6, provided by the Vegetable Research Institute of Guangxi Academy of Agricultural Sciences.
[0032] 2. Test methods and results
[0033] (1) Cultivate seedlings
[0034] Soak the seeds in clean water for 6-7 hours, rinse them off, wrap them with clean moist gauze and place them at a constant temperature at 30°C to accelerate germination, place them on the same plug at intervals of different materials, and manage the seedlings as normal.
[0035] (2) Determination of physiological and biochemical indicators under low temperature stress
[0036] The seedlings with 4 leaves and 1 heart were placed under the condition of 10°C for cold stress treatment, and the functional leaves of bitter gourd were taken at treatment 0d, 2d, 4d, 6d, 8d, and 10d, and the soluble protein content, proline Determination of physiological and biochemical in...
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The invention discloses a method for selecting the material collecting period for cold tolerance authentication in a momordica charantia L. seedling period. Materials which are authenticated and have cold tolerance differences are utilized for low-temperature stress treatment, functional leaves in different stress periods are taken to be subjected to measurement of physiological and biochemical indexes, data processing results are analyzed, the known cold tolerance of test materials is combined, so that the suitable material collecting period for the cold tolerance authentication in the momordica charantia L. seedling period by utilizing a physiological and biochemical index method is determined. According to the method, momordica charantia L. is authenticated and evaluated by utilizing the authentication indexes in the low-temperature stress proper period, the authenticating and evaluating reliability and the authenticating and evaluating accuracy are improved, the result is suitable for the authentication of the cold tolerance of the seedling periods of different momordica charantia L. resources, and the method lays the foundation for the cold tolerance breeding of momordica charantia L..
Description
technical field [0001] The invention belongs to the technical field of identification and evaluation of balsam pear germplasm resources, and in particular relates to a method for selecting a material collection period for identification of cold tolerance at the seedling stage of balsam pear. Background technique [0002] Bitter gourd (Momordicacharantia L.) originated in the tropical region of Southeast Asia, and is widely distributed in tropical, subtropical and temperate regions. In case of low temperature and cold weather, the growth is often slow, and after a long time of stress, it will form stiff seedlings and stop growing, which limits the planting range and market season of bitter gourd, and restricts the development of bitter gourd industry. [0003] To carry out cold-tolerant breeding of bitter gourd, the first thing is to identify and evaluate the cold-tolerant breeding resources. Physiological and biochemical index methods are often used in the identification an...
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IPC IPC(8): A01G1/00A01C1/00A01G7/06
CPCA01C1/00A01G7/06
Inventor 陈小凤黄如葵陈振东黄玉辉梁家作黄熊娟冯诚诚刘杏连秦健
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI