A kind of preparation method of environment improving agent at the bottom of aquaculture water body
A technology for aquaculture water and improver, applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc. High problems, to achieve the effect of fast growth and reproduction, obvious effect, and good absorption
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Embodiment 1
[0019] (1) Strain recovery: Pick bacteria from a single colony of the standard Enterococcus faecalis strain and inoculate it into the Enterococcus faecalis culture medium, and culture it at 35°C for 12 hours.
[0020] (2) Bacteria enrichment: inoculate in Enterococcus faecalis culture medium with 4% volume ratio again, under 35 ℃ of conditions, carry out fermentation, cultivate 28h, reach 10 to the concentration of bacterium suspension bacterium 8 fu / g; Enterococcus faecalis fermentation broth was obtained;
[0021] Wherein, described enterococcus faecalis culture medium, its preparation method is as follows: tryptone 10g / L, yeast powder 10g / L, glucose 10g / L, anhydrous calcium chloride 0.04g / L, magnesium sulfate heptahydrate 0.019 2g / L L, dipotassium hydrogen phosphate 0.04g / L, potassium dihydrogen phosphate 0.04g / L, sodium hydrogen phosphate 0.4g / L, sodium chloride 0.08g / L, potato juice 50g / L, calcium carbonate 1g / L, sodium acetate 10g / L, ammonium carbonate 2g / L, ammonium ac...
Embodiment 2
[0024] (1) Strain recovery: Pick bacteria from a single colony of the standard Enterococcus faecalis strain and inoculate it into the Enterococcus faecalis culture medium, and culture it at 35°C for 12 hours.
[0025](2) Bacteria enrichment: inoculate in Enterococcus faecalis culture medium with 4% volume ratio again, under 35 ℃ of conditions, carry out fermentation, cultivate 28h, reach 10 to the concentration of bacterium suspension bacterium 8 fu / g; Enterococcus faecalis fermentation broth was obtained;
[0026] Wherein, described enterococcus faecalis culture medium, its preparation method is as follows: tryptone 10g / L, yeast powder 10g / L, glucose 10g / L, anhydrous calcium chloride 0.04g / L, magnesium sulfate heptahydrate 0.019 2g / L L, dipotassium hydrogen phosphate 0.04g / L, potassium dihydrogen phosphate 0.04g / L, sodium hydrogen phosphate 0.4g / L, sodium chloride 0.08g / L, potato juice 50g / L, calcium carbonate 1g / L, sodium acetate 10g / L, ammonium carbonate 2g / L, ammonium ace...
Embodiment 3
[0029] (1) Strain recovery: Pick bacteria from a single colony of the standard Enterococcus faecalis strain and inoculate it into the Enterococcus faecalis culture medium, and culture it at 35°C for 12 hours.
[0030] (2) Bacteria enrichment: inoculate in Enterococcus faecalis culture medium with 4% volume ratio again, under 35 ℃ of conditions, carry out fermentation, cultivate 48h, reach 10 to the concentration of bacterium suspension bacterium 9 fu / g; Enterococcus faecalis fermentation broth was obtained;
[0031] Wherein, described enterococcus faecalis culture medium, its preparation method is as follows: tryptone 10g / L, yeast powder 10g / L, glucose 10g / L, anhydrous calcium chloride 0.04g / L, magnesium sulfate heptahydrate 0.019 2g / L L, dipotassium hydrogen phosphate 0.04g / L, potassium dihydrogen phosphate 0.04g / L, sodium hydrogen phosphate 0.4g / L, sodium chloride 0.08g / L, potato juice 50g / L, calcium carbonate 1g / L, sodium acetate 10g / L, ammonium carbonate 2g / L, ammonium ac...
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