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A kind of preparation method of environment improving agent at the bottom of aquaculture water body

A technology for aquaculture water and improver, applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc. High problems, to achieve the effect of fast growth and reproduction, obvious effect, and good absorption

Active Publication Date: 2017-05-24
惠雪(广州)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

3. Ion exchange type: products mainly containing EDTA or sodium thiosulfate are used to reduce positive harmful substances of ammonia nitrogen heavy metals in water or at the bottom, or to positively oxidize bromine, chlorine and iodine compounds, potassium permanganate, etc. It is used for detoxification when substance poisoning, and the effect is relatively ideal, but the effect is very poor for negatively charged acidic harmful substances in water or the bottom layer
4. Degradation type of live bacteria: use the biological substrate modifier of Bacillus subtilis, Bacillus natto, photosynthetic bacteria and other fungi to decompose the bottom organic matter through the decomposition of biological bacteria. The disadvantage is that the bottom layer is in an anoxic state and the decomposition ability The survival rate of strong Bacillus, etc. is not high, and it is very likely to accelerate the hypoxia at the bottom of the pond
All these treatment methods are used in the form of granules or powders. Powders mainly function in water bodies, and it is difficult to reach the bottom, so the improvement effect on the bottom is limited.
Although the particle form can reach the bottom, it is relatively fixed at the bottom of a small area after reaching the bottom. Although it can improve the bottom to a certain extent, it is only partially improved, and cannot comprehensively improve the culture substrate. Improve

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Strain recovery: Pick bacteria from a single colony of the standard Enterococcus faecalis strain and inoculate it into the Enterococcus faecalis culture medium, and culture it at 35°C for 12 hours.

[0020] (2) Bacteria enrichment: inoculate in Enterococcus faecalis culture medium with 4% volume ratio again, under 35 ℃ of conditions, carry out fermentation, cultivate 28h, reach 10 to the concentration of bacterium suspension bacterium 8 fu / g; Enterococcus faecalis fermentation broth was obtained;

[0021] Wherein, described enterococcus faecalis culture medium, its preparation method is as follows: tryptone 10g / L, yeast powder 10g / L, glucose 10g / L, anhydrous calcium chloride 0.04g / L, magnesium sulfate heptahydrate 0.019 2g / L L, dipotassium hydrogen phosphate 0.04g / L, potassium dihydrogen phosphate 0.04g / L, sodium hydrogen phosphate 0.4g / L, sodium chloride 0.08g / L, potato juice 50g / L, calcium carbonate 1g / L, sodium acetate 10g / L, ammonium carbonate 2g / L, ammonium ac...

Embodiment 2

[0024] (1) Strain recovery: Pick bacteria from a single colony of the standard Enterococcus faecalis strain and inoculate it into the Enterococcus faecalis culture medium, and culture it at 35°C for 12 hours.

[0025](2) Bacteria enrichment: inoculate in Enterococcus faecalis culture medium with 4% volume ratio again, under 35 ℃ of conditions, carry out fermentation, cultivate 28h, reach 10 to the concentration of bacterium suspension bacterium 8 fu / g; Enterococcus faecalis fermentation broth was obtained;

[0026] Wherein, described enterococcus faecalis culture medium, its preparation method is as follows: tryptone 10g / L, yeast powder 10g / L, glucose 10g / L, anhydrous calcium chloride 0.04g / L, magnesium sulfate heptahydrate 0.019 2g / L L, dipotassium hydrogen phosphate 0.04g / L, potassium dihydrogen phosphate 0.04g / L, sodium hydrogen phosphate 0.4g / L, sodium chloride 0.08g / L, potato juice 50g / L, calcium carbonate 1g / L, sodium acetate 10g / L, ammonium carbonate 2g / L, ammonium ace...

Embodiment 3

[0029] (1) Strain recovery: Pick bacteria from a single colony of the standard Enterococcus faecalis strain and inoculate it into the Enterococcus faecalis culture medium, and culture it at 35°C for 12 hours.

[0030] (2) Bacteria enrichment: inoculate in Enterococcus faecalis culture medium with 4% volume ratio again, under 35 ℃ of conditions, carry out fermentation, cultivate 48h, reach 10 to the concentration of bacterium suspension bacterium 9 fu / g; Enterococcus faecalis fermentation broth was obtained;

[0031] Wherein, described enterococcus faecalis culture medium, its preparation method is as follows: tryptone 10g / L, yeast powder 10g / L, glucose 10g / L, anhydrous calcium chloride 0.04g / L, magnesium sulfate heptahydrate 0.019 2g / L L, dipotassium hydrogen phosphate 0.04g / L, potassium dihydrogen phosphate 0.04g / L, sodium hydrogen phosphate 0.4g / L, sodium chloride 0.08g / L, potato juice 50g / L, calcium carbonate 1g / L, sodium acetate 10g / L, ammonium carbonate 2g / L, ammonium ac...

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PUM

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Abstract

The invention provides a preparation method for an aquaculture water bottom environment modifier. According to the preparation method, disinfected and sterilized high water-absorbent resin is put into a 10<8>-10<9> cfu / g of an enterococcus faecalis fermentation solution; through 2-4 hours' soaking, the enterococcus faecalis fermentation solution can be sufficiently adsorbed by the high water-absorbent resin; 100-300 g of the high water-absorbent resin corresponds to 2000-3000 ml of the enterococcus faecalis fermentation solution. When the enterococcus faecalis fermentation solution is sufficiently adsorbed by the high water-absorbent resin, the high water-absorbent resin adsorbing the enterococcus faecalis fermentation solution is subpackaged into cans of 500 g or 1000 g under the sterile condition, and finally the cans are filled into boxes for storage. Through adoption of the modifier obtained according to the preparation method, the defects that the conventional bottom environment modifying product used in the current market cannot sink to the bottom of a pool and fully improve the bottom environment at the pool bottom are overcome, secondary water pollution is prevented, and a good bottom environment is created for aquiculture organisms.

Description

technical field [0001] The invention belongs to the technical field of aquaculture. The invention relates to a preparation method of a penaeus vannamei culture water pull-net type biological substrate improving agent, in particular to a comprehensive effect and pull-net type bottom environment improvement of the culture water body. Background technique [0002] Penaeus vannamei is characterized by fast growth, strong adaptability, tender meat, high rate of processed meat, long survival time out of water and strong disease resistance. Therefore, the aquaculture area in my country is developing rapidly. million hectares. However, in recent years, with the popularization and popularization of intensive and high-density farming methods, the water environment for shrimp farming has deteriorated day by day, and the pH, chemical oxygen demand (COD), total inorganic nitrogen (TIN), and other indicators of the farming water have seriously exceeded the standard. Penaeus white shrimp ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34C02F3/28
Inventor 骆桂兰高嘉泰陈军王会聪王煜恒曹寒
Owner 惠雪(广州)生物科技有限公司
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