Dual PCR method for detecting theileria hirci and anaplasma

Abstract

The invention relates to a dual PCR method for detecting theileria hirci and anaplasma. The method includes the steps of finding a gene conserved seuqnece target gene locus and designing a specific primer sequence I according to the 18S rRNA gene sequence of theileria hirci, finding a gene conserved seuqnece target gene locus and designing the specific primer sequence II according to the 16S rRNA gene sequence of anaplasma, adding the upstream primer and the downstream primer of the specific primer sequence I and the upstream primer and the downstream primer of the specific primer sequence II and PCR mixed liquid into a PCR pipe, mixing the materials and adding theileria hirci DNA and anaplasma DNA extracted from a to-be-tested blood sample to the mixture, putting the PCR pipe into a PCR amplification instrument to be circulated, obtaining a PCR product, and putting the PCR product on 2% sepharose gel to be subjected to electrophoresis. The method has the advantages of being rapid, specific, sensitive, efficient, low in cost and the like and is beneficial to clinical application.

Description

technical field [0001] The invention belongs to the field of molecular biology, in particular to a method for detecting Theileria sheep ( Theileria spp) and slurry-free ( Anaplasma spp) double PCR method. technical background [0002] Theileriasis of sheep is a tick-borne blood protozoan disease of sheep and goats caused by the pathogen of the genus Theileria ovis. The disease was first discovered in sheep in Egypt by Jittlewood in 1914, and was first reported in China by Yang Fuguo et al. In some areas, the infection rate of sheep Theileria is as high as 95%. The diseased sheep show symptoms such as high fever, enlarged lymph nodes on the body surface, anemia and emaciation, and even death in severe cases, which has caused great harm to the sheep breeding industry in my country. Sheep anplasmosis (commonly known as anaplasmosis) is a tick-borne hematological rickettsial disease caused by a pathogen of the genus Anaplasma. When sheep and goats are infected, the main mani...

AI Technical Summary

Problems solved by technology

[0006] Aiming at the problems of low detection rate and cumbersome operation of the current detection methods for Theileria ovis and Aplasma, the present invention establishes a method that can simultaneously Rapid test for detection of Theileria sheep ( Theileria spp) and slurry-free ( Anaplasma spp) double PCR method

Images