Constant temperature antibody incubation box

Abstract

The invention discloses a constant temperature antibody incubation box. The constant temperature antibody incubation box comprises an insulation box body 1, a box cover 2 and a sealing ring 34. A plurality of baffles 4, an inner cavity 5 and an inner cavity cover 6 are arranged in the insulation box body 1. The insulation box body 1 and the box cover 2 realize the effects of sealing, preventing liquid evaporation and liquid leakage through a sealing ring 3. The height of the baffle 4 is smaller than that of the insulation box body 1, but is greater than or equal to one half of that of the insulation box body 1. The baffles 4 can be directly connected or not connected with the outer cavity wall of the inner cavity 5. The constant temperature antibody incubation box has the benefits that the inner cavity and the inner cavity cover which can be tightly buckled to each other are arranged in the insulation box body, constant temperature incubation can be guaranteed by laying ice water mixture or materials with high cold storage capacity in the box, degradation of antibodies is avoided, and the experimental reproducibility is improved; cylindrical insulation box body design effectively prevents membranes or slices from being adhered to the insulation box body, and insufficient incubation of the membranes or the slices is avoided; an incubation space can be adjusted in a manner of adjusting the quantity of baffles and adjusting the baffles to be connected or not connected with the outer wall of the inner cavity, and the quantity of disposable incubation membranes or slices is increased.

Description

technical field [0001] The invention relates to an incubation device in the fields of biotechnology and medical care, in particular to a constant temperature antibody incubation device. Background technique [0002] Western blotting and immunohistochemistry are the most commonly used techniques in molecular biology experiments and clinical tumor pathological diagnosis. In western blotting experiments, after proteins are transferred and fixed on nitrocellulose or nylon membranes by electrophoresis and membrane transfer techniques, the membranes need to be co-incubated with a specific antibody solution, and then rinsed and developed. The binding of antibodies to specific antigenic components in proteins can be observed, and further used to detect changes in the content of specific proteins in samples. In immunohistochemical experiments, it is also necessary to co-incubate tissue sections with antibody solutions, and then perform steps such as rinsing and color development to ...

AI Technical Summary

Problems solved by technology

However, the currently used incubator box has no cold storage capacity, and the incubation effect is not ideal

If the antibody incubation box is placed in a crushed ice shaker, the melted ice water will easily seep into the incubation solution, which will affect the experimental results

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