Unlock instant, AI-driven research and patent intelligence for your innovation.

Macro-element nutrient salt formula for large-scale culture of Thalassiossira sp.

A large-scale culture, a large number of elements technology, applied in the field of aquaculture, can solve the problems affecting the seedling process and seedling quality, unstable cell nutrients, unable to solve the problem of sea chain algae, etc., to reduce the cost of breeding and the stability of cell nutrients. , cultivating a long-lasting effect

Inactive Publication Date: 2015-12-30
ZHANJIANG HENGXING SOUTHERN OCEAN TECH
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

F / 2 medium has disadvantages such as easy contamination and low culture density, which can easily lead to low culture efficiency and unstable nutrient composition of the cultured cells, which seriously affects the seedling cultivation process and quality
[0005] Existing technologies fail to address this shortcoming in the Thalassiosira cultivation process

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 The following steps were used for large-scale cultivation of Thalassiosira:

[0022] (1) Will be used for an area of ​​200m 2 The track-type bioreactor is thoroughly cleaned and disinfected, and then the seawater that has been subjected to sand filtration, foam separation, precision filter and ultraviolet disinfection is introduced. The depth of the seawater is 25cm. Then, the strong chlorine essence is added to the seawater at a dose of 20ppm for disinfection. And aeration all day and night;

[0023] (2) After the seawater is treated in step (1), add sodium thiosulfate at a dose of 30 ppm;

[0024] (3) According to the following formula, weigh each component according to the volume of seawater, and 3 The number of g of this component contained in seawater: NaNO 3 60g / m 3 , NH 4 HCO 3 80g / m 3 , Na 2 HPO 4 10g / m 3 , FeCl 3 2.5g / m 3 , EDTA-2Na9.5g / m 3 , NaSiO 3 20g / m 3 ;

[0025] (4) Add each component to the sea water, turn on the rotor and stir at 80r / min for 5 minute...

Embodiment 2

[0027] Example 2 The following steps were used for large-scale cultivation of Thalassiosira:

[0028] (1) Will be used for an area of ​​200m 2 The track-type bioreactor is thoroughly cleaned and disinfected, and then the seawater that has been subjected to sand filtration, foam separation, precision filter and ultraviolet disinfection is introduced. The depth of the seawater is 30cm, and then strong chlorine essence is added to the seawater at a dose of 20ppm for disinfection. And aeration all day and night;

[0029] (2) After the seawater is treated in step (1), add sodium thiosulfate at a dose of 30 ppm;

[0030] (3) According to the following formula, weigh each component according to the volume of seawater, and 3 The number of g of this component contained in seawater: NaNO 3 58g / m 3 , NH 4 HCO 3 78g / m 3 , Na 2 HPO 4 11g / m 3 , FeCl 3 2.8g / m 3 , EDTA-2Na10g / m 3 , NaSiO 3 22g / m 3 ;

[0031] (4) Add each component to the sea water, turn on the rotor and stir at 80r / min for 10 minutes...

Embodiment 3

[0033] Example 3 The following steps were used for large-scale cultivation of Thalassiosira:

[0034] (1) Will be used for an area of ​​200m 2 The track-type bioreactor is thoroughly cleaned and disinfected, and then the seawater that has been subjected to sand filtration, foam separation, precision filter and ultraviolet disinfection is introduced. The depth of the seawater is 25cm. Then, the strong chlorine essence is added to the seawater at a dose of 20ppm for disinfection. And aeration all day and night;

[0035] (2) After the seawater is treated in step (1), add sodium thiosulfate at a dose of 30 ppm;

[0036] (3) According to the following formula, weigh each component according to the volume of seawater, and 3 The number of g of this component contained in seawater: NaNO 3 62g / m 3 , NH 4 HCO 3 83g / m 3 , Na 2 HPO 4 9g / m 3 , FeCl 3 2.6g / m 3 , EDTA-2Na9.8g / m 3 , NaSiO 3 18g / m 3 ;

[0037] (4) Add each component to the seawater, turn on the rotor and stir at a speed of 80r / min. At...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a macro-element nutrient salt formula for large-scale culture of Thalassiossira sp. The nutrient salt formula comprises the following components in content by gram per m<3> of seawater: 55 to 65g / m<3> of NaNO3, 75 to 86g / m<3> of NH4HCO3, 7 to 12 g / m<3> of Na2HPO4, 2.2 to 3.0g.m<3> of FeCl3, 9.0 to 10.5g / m<3> of EDTA-2Na and 16 to 24g / m<3> of NaSiO3. The macro-element nutrient salt formula for large-scale culture of Thalassiossira sp. has the advantages that the growth of Thalassiossira sp. can be promoted, the culture density is improved, the culture efficiency is improved and the cell nutrient components of the obtained Thalassiossira sp. are stable, further has the characteristics of high culture density, long duration and low cost, and compared with the prior art, can obviously improve the cell density of the Thalassiossira sp. and obviously prolong the culture time of the Thalassiossira sp.

Description

Technical field [0001] The invention belongs to the field of aquaculture, and particularly relates to a macroelement nutrient salt formula suitable for large-scale cultivation of Thalassiosira. Background technique [0002] As a renewable biomass energy source, microalgae has also received more and more attention. There have also been related studies and progress in the industrialization of large-scale cultivation of microalgae. However, due to the fact that large-scale cultivation of microalgae is susceptible to light, cultivation conditions and other factors The existing microalgae industrialization model still has the disadvantages of low efficiency and high cost. [0003] Thalassiosira ( Thalassiossira sp.) is a diatom ( Bacillariophyta ), Diatoms ( Centrales ), Discoid Diatom ( Discoideae ), Thalassiosiraceae ( Thalassiosoraceae ), Thalassiosira ( Thalassiossira ), usually connected by glial filaments at the edge of the shell to form a chain group (4-12 cells), the length...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/12
Inventor 李色东刘涛胡一承
Owner ZHANJIANG HENGXING SOUTHERN OCEAN TECH