Testing reagent for activated partial thromboplastin time and preparation method of testing reagent

Abstract

The invention relates to a testing reagent for activated partial thromboplastin time. The testing reagent is prepared from phospholipids, activator and stabilizer. The stabilizer is prepared from, by mass, 1%-4% of alanine and 0.5-5 mM of catechin. A preparation method of the testing reagent includes the steps that an HEPES buffer solution is prepared, the pH value of the buffer solution is adjusted to be neutral, and sodium chloride is added; the activator is added to the buffer solution, phospholipids and other components are added after stirring and even mixing are conducted, and then the testing reagent for APTT is obtained after even mixing, stirring and filtering are conducted. According to the testing reagent for activated partial thromboplastin time, stabilizer components with high toxicity are removed, so that production and use processes are safer; meanwhile, the stability and detection accuracy of the testing reagent for APTT are greatly improved.

Description

technical field [0001] The invention relates to a coagulation reagent and a preparation method thereof, in particular to an activated partial thromboplastin time determination reagent and a preparation method thereof. Background technique [0002] Activated partial thromboplastin time (APTT) can be used to judge the state of endogenous coagulation activity, and is one of the main clinical coagulation performance test indicators. The main components of the activated partial thromboplastin time assay reagent are activator and phospholipids, which are lipids containing phosphoric acid and belong to complex lipids. As platelet substitutes, phospholipids participate in the intrinsic coagulation pathway. As one of the main components of activated partial thromboplastin time assay reagents, phospholipids are rich in unsaturated fatty acids, which are easily oxidized to produce a series of lipid peroxidation, which affects its physiological activity. Taking phosphatidylcholine as ...

AI Technical Summary

Problems solved by technology

Among these commonly used stabilizers, butylated hydroxytoluene has certain toxicity and is relatively less toxic, while sodium azide is more toxic and is easy to detonate when it comes into contact with copper pipes, etc. Therefore, the use of raw materials in the production process is increased. and control difficulty

In addition, in addition to adding butylated hydroxytoluene or butylated hydroxyanisole, sodium azide, in order to further improve the stability of the activated partial thrombin assay reagent, polysaccharide or polyol polymers are added to the reagent (JP2994557B2, JP3330685B2) , the addition of such substances can improve the dispersibility and uniformity of the composition contained in APTT, especially the activator, or as a suspending agent, increase the viscosity of the solution, so that the colloidal particles can be suspended in the solution for a long time without sedimentation, However, the addition of this type of polymer compound will reduce the accuracy of the APTT value measured by the optical method while increasing the viscosity of the mixed liquid.

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