Method for virus-free and rapid propagation of rhizoma atractylodis macrocephalae

A technique for Atractylodes Rhizoma and virus-free seedlings, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of serious virus accumulation, decreased yield and quality, lack of species purification and rejuvenation, etc., to improve the survival rate and prevent diseases. The occurrence, economical and practical effect of the method

Inactive Publication Date: 2016-01-06
耿跃
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to preserve high-quality varieties, increase yield and shorten the growth cycle, Yaonong adopts asexual reproduction in the cultivation of Atractylodes mac

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for detoxification and rapid propagation of Atractylodes macrocephala is characterized in that it mainly includes the following operating and processing steps:

[0022] (1) Basic training:

[0023] Select seeds with full grains and no disease, soak them in normal temperature water for 24 hours, layer them, cover them with a damp cloth for 4-6 days, and control the temperature at 21-27°C. After the seeds germinate, take the bud tips as explants. Soak in 70% alcohol solution under sterile conditions for 8 seconds, then soak in 0.1% mercuric solution for 10 minutes, soak in sterile water for 6 to 7 times, and carry out basic culture of the bud tip of Atractylodes macrocephala, the formula of the basic medium The components are: MS+6-BA0.65mg / L, NAA0.13mg / L, maltose 10g / L, agar 3g / L;

[0024] (2), detoxification culture:

[0025] Take the shoot tips of Atractylodes macrocephala after the above-mentioned basic culture, and carry out detoxification culture. After t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for virus-free and rapid propagation of rhizoma atractylodis macrocephalae and belongs to the technical field of agricultural propagation. The method mainly comprises the operation processing steps that (1) full seeds without diseases are selected for accelerating germination, shoot tips of the seeds are used as explants, and embryos of rhizoma atractylodis macrocephalae are subjected to foundation culture; (2) the embryos, subjected to foundation culture, of rhizoma atractylodis macrocephalae are subjected to virus-free culture, obtained tissue culture seedlings are heated at the temperature of 40 DEG C for 30 days, 0.2cm stem tips are stripped, and obtained stem tip test-tube plantlets are virus-free seedlings of rhizoma atractylodis macrocephalae; (3) the virus-free seedlings of rhizoma atractylodis macrocephalae are subjected to multiplication tissue culture rapid propagation; (4) rooting culture is conducted, wherein the tissue cultured virus-free seedlings of rhizoma atractylodis macrocephalae are subjected to rooting propagation; (5) rooted seedlings of rhizoma atractylodis macrocephalae are moved under strong illumination for hardening for three days, transplanted to a matrix containing soil, pleurotus eryngii and vermiculite according to the proportion of 1:1:1 and then transplanted to fields after growth is stable. The method for virus-free and rapid propagation of rhizoma atractylodis macrocephalae is suitable for industrial seedling growing, the survival rate of seedling growing is greatly improved, occurrence of diseases is effectively prevented, and the method is economical and practical.

Description

technical field [0001] The invention relates to the technical field of agricultural breeding, in particular to a method for detoxification and rapid propagation of Atractylodes macrocephala. Background technique [0002] Atractylodes Rhizoma Atractylodes is a perennial herb belonging to the Asteraceae family. It likes a cool climate, and its rhizomes are used as medicine, which has many medicinal functions. It is mainly distributed in mountainous wetlands such as Sichuan, Yunnan, and Guizhou. It is an important Chinese herbal medicine, and the traditional planting method is single-furrow planting in flakes. [0003] The traditional propagation method of Atractylodes macrocephala is to use seeds for sexual reproduction, sowing seedlings in the first year, cultivating strong seedlings, and transplanting in the second year. In order to preserve high-quality varieties, increase production and shorten the growth cycle, Yaonong cultivated Atractylodes macrocephala by asexual re...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00
Inventor 耿跃
Owner 耿跃
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap