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Compound for inhibiting growth of burkholderia glumae

A kind of rice blight bacteria, bacterial technology, applied in the field of antibacterial and bactericidal activity of rice bacterial blight blight, can solve the problems of rice seedling rot epidemic and other problems

Inactive Publication Date: 2016-01-06
许自协
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Before the 1970s, the disease was always a secondary disease on rice in Japan. However, in the late 1970s, due to the implementation of mechanized production in Japan and the large-scale industrial seedling raising, rice seedling rot became popular and became the most serious disease on rice in Japan. one of the diseases

Method used

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  • Compound for inhibiting growth of burkholderia glumae
  • Compound for inhibiting growth of burkholderia glumae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The minimum inhibitory concentration (MIC) value was measured.

[0014] (1) Preparation of nutrient broth: take 30g of nutrient broth and add 1000mL of distilled water. Sterilize by autoclaving at 121°C for 20min before use.

[0015] (2) Preparation of nutrient agar solid medium: take 45 g of nutrient agar and add 1000 mL of distilled water. Sterilize by autoclaving at 121°C for 20min before use.

[0016] (3) Cultivation of strains: The operation is carried out on an ultra-clean workbench. Draw 10 mL of sterilized liquid medium, put it in a sterilized test tube, then pick a colony with a bacterial ring, add it to the liquid medium, and put it in the incubator for culturing. The bacteria are cultured for 24 hours at a temperature of 28°C.

[0017] (4) Bacterial liquid preparation and counting: the cultured bacterial liquid is diluted with liquid medium by 10-fold dilution method, and is preliminarily observed and counted on a microscope with a hemocytometer, and then...

Embodiment 2

[0024] Measure the minimum bactericidal concentration MBC value.

[0025] (1) Preparation of nutrient broth: take 30g of nutrient broth and add 1000mL of distilled water. Sterilize by autoclaving at 121°C for 20min before use.

[0026] (2) Preparation of nutrient agar solid medium: take 45 g of nutrient agar and add 1000 mL of distilled water. Sterilize by autoclaving at 121°C for 20min before use.

[0027] (3) Cultivation of strains: The operation is carried out on an ultra-clean workbench. Draw 10 mL of sterilized liquid medium, put it in a sterilized test tube, then pick a colony with a bacterial ring, add it to the liquid medium, and put it in the incubator for culturing. The bacteria are cultured for 24 hours at a temperature of 28°C.

[0028] (4) Bacterial liquid preparation and counting: Dilute the cultured bacterial liquid with liquid medium by 10-fold dilution method, and use a hemocytometer to preliminarily observe and count on a microscope, then dilute the bacte...

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Abstract

The invention discloses a compound having inhibitory and bactericidal activities on burkholderia glumae. An MIC (Minimal Inhibitory Concentration) value of the burkholderia glumae is 0.5 microgram / mL, and an MBC (Minimal Bactericidal Concentration) value is 1 microgram / mL.

Description

technical field [0001] The invention relates to a new application of the compound, clarifies the relationship between the chemical structure of the medicine and the biological activity of the bacteria, and more specifically explores the antibacterial and bactericidal activities of the compound on rice bacterial rice blight. Background technique [0002] Rice bacterial grain blight (also known as rice bacterial glume blight) is a serious seed-borne disease that mainly harms rice. Leaf inoculation shows that oats and other gramineous crops, Compositae, leguminous Plants such as Polygonaceae and Plantaceae also showed susceptibility. [0003] Rice bacterial grain blight was first discovered in Kyushu, Japan in 1956. In 1967, Kurita and Tabei named the pathogen Pseudomonas gliaculum for the first time, and in 1994 it was renamed Burkholderia gliatum. The rice disease caused was first discovered in the 1950s as a symptom of grain rot, so it was named bacterial grain blight, but ...

Claims

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Application Information

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IPC IPC(8): A01N43/86A01P1/00
Inventor 不公告发明人
Owner 许自协