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Application of endophytic bacteria b29 in Sophora japonica in the control of Panax notoginseng anthracnose

An endogenous bacteria and anthracnose technology, applied in the direction of bacteria, application, and microbial-based methods, can solve problems such as pollution, health threats, and pesticide residues in Panax notoginseng products, and achieve broad application prospects and strong inhibitory effects

Active Publication Date: 2019-07-16
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the traditional authentic production area of ​​Sanqi in Guangxi, due to the low altitude and high temperature and humidity weather conditions, this disease often occurs at the same time, resulting in a large amount of production reduction or even no harvest of Sanqi, which has brought huge economic losses to the growers.
In order to control this fungal disease, a large number of chemical pesticides are used, resulting in a large number of pesticide residues in Sanqi products, which seriously pollute the environment and greatly threaten people's health

Method used

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  • Application of endophytic bacteria b29 in Sophora japonica in the control of Panax notoginseng anthracnose
  • Application of endophytic bacteria b29 in Sophora japonica in the control of Panax notoginseng anthracnose
  • Application of endophytic bacteria b29 in Sophora japonica in the control of Panax notoginseng anthracnose

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Isolation, screening and identification of strains

[0023] 1. Isolation of strains

[0024] Test material: wild Vietnamese pagoda tree collected from the limestone area of ​​Tiandeng County, Guangxi.

[0025] Tested strains: provided by Institute of Plant Pathology, Guangxi University.

[0026] Medium: 1000ml NA medium: beef extract 3g, yeast extract 1g, peptone 5g, sucrose 10g, agar 15g, pH 7.0.

[0027] Surface disinfection: Rinse the fresh and healthy Vietnamese Sophorae root with a length of 6-8cm and a width of 1-2cm with running water for 30 minutes to wash away the sediment, then rinse the washed Vietnamese Sophorae root with sterile water twice, and air-dry the surface moisture , moved to the ultra-clean workbench, and under aseptic conditions, the Sophora japonica root was soaked in 75% ethanol for 1 min, rinsed with sterile water twice, soaked in sodium hypochlorite (available chlorine 1%) for 2 min, washed with sterile water for 3 times , dry the surface ...

Embodiment 2

[0079] Inhibitory effect of metabolites of Sophora japonica endophytic bacteria B29 on Panax notoginseng anthracnose 1. Fermentation culture of strains and extraction of metabolites

[0080] Inoculate the endophytic bacteria B29 of Sophora vietnamese into a 2-liter Erlenmeyer flask containing 1000ml of NB liquid medium (3g of beef extract, 1g of yeast extract, 5g of peptone, 10g of sucrose, pH 7.0), and place it at a temperature of 28°C at 130r / min shaking table fermentation culture for 10 days, the obtained fermented product was ultrasonically ultrasonicated for 40min, and then extracted twice with ethyl acetate, and the obtained ethyl acetate phase was concentrated under reduced pressure twice to obtain the crude ethyl acetate extraction of the fermented product of the strain The product is the metabolite of endophytic bacteria B29 in Sophora vietnamese.

[0081] 2. Inhibitory effect of endophytic B29 metabolites of Sophora japonica on mycelial growth of Panax notoginseng an...

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Abstract

The invention discloses an endophytic bacterium B29 of Sophora japonica. The classification of the endophytic bacterium B29 of Sophora japonica is named as Burkholderia sp. B29, and the 16S rDNA gene sequence table of the strain is shown as SEQ ID NO.1 As stated, depository unit: General Microbiology Center of China Microbial Culture Collection Management Committee, deposit address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, deposit date: May 13, 2015, deposit number : CGMCC No.10807. The present invention isolates and screens an endophytic bacterium (Burkholderia sp.) B29 for the first time from the root of the medicinal plant Phytophthora japonica. Bring broad application prospects.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of a kind of Sophora japonica endophytic bacterium B29 in the prevention and treatment of Panax notoginseng anthracnose. Background technique [0002] The prevention and control of plant diseases in modern agriculture is overly dependent on the use of chemical pesticides. The application of a large number of chemical pesticides not only has long-term damage to the ecological environment, but also causes the quality of agricultural products to decline, pesticide residues exceed the standard, pathogenic bacteria are resistant to drugs, and are harmful to humans and animals. Many questions. It is of great significance to find safer and more effective disease control methods, and using biological methods to prevent and control plant diseases can effectively solve the above problems. [0003] Sanqi (Panax notoginseng F.H.chen), also known as Tianqi, Jinbuhuan, etc., has r...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/04A01N63/02A01P3/00C12R1/01
CPCA01N63/10C12P1/04C12N1/205C12R2001/01
Inventor 黄荣韶姚裕群蓝芳李良波
Owner GUANGXI UNIV