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Compound for suppressing growth of phialophora gregata

A brown rot fungus and compound technology, applied in the field of compounds, can solve problems such as blockage and catheter discoloration

Inactive Publication Date: 2016-01-06
许自协
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mycelia extend in host xylem vessels, causing vessel discoloration and blockage

Method used

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  • Compound for suppressing growth of phialophora gregata
  • Compound for suppressing growth of phialophora gregata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Measure the minimum inhibitory concentration MIC value.

[0016] (1) Preparation of nutrient broth: Take 30g of nutrient broth and add 1000mL of distilled water. Sterilize by high pressure steam at 121°C for 20 minutes before use.

[0017] (2) Preparation of nutrient agar solid medium: take 45g of nutrient agar and add 1000mL of distilled water. Sterilize by high pressure steam at 121°C for 20 minutes before use.

[0018] (3) Cultivation of bacterial strains: the operation is carried out on a clean bench. Draw 10mL of sterilized liquid culture medium, put it in a sterilized test tube, then pick a colony with the inoculation loop, add it to the liquid medium, and put it in the incubator for cultivation. The bacteria are cultivated for 24 hours at a temperature of 20°C.

[0019] (4) Bacterial solution preparation and counting: Dilute the cultured bacterial solution with liquid medium by 10-fold dilution method, and use a hemocytometer to observe and count initially on...

Embodiment 2

[0026] Measure the minimum bactericidal concentration MBC value.

[0027] (1) Preparation of nutrient broth: Take 30g of nutrient broth and add 1000mL of distilled water. Sterilize by high pressure steam at 121°C for 20 minutes before use.

[0028] (2) Preparation of nutrient agar solid medium: take 45g of nutrient agar and add 1000mL of distilled water. Sterilize by high pressure steam at 121°C for 20 minutes before use.

[0029] (3) Cultivation of bacterial strains: the operation is carried out on a clean bench. Draw 10mL of sterilized liquid culture medium, put it in a sterilized test tube, then pick a colony with the inoculation loop, add it to the liquid medium, and put it in the incubator for cultivation. The bacteria are cultivated for 24 hours at a temperature of 20°C.

[0030] (4) Bacterial solution preparation and counting: Dilute the cultured bacterial solution with liquid medium by 10-fold dilution method, and use a hemocytometer to observe and count initially ...

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Abstract

The invention discloses a compound for suppressing the growth of phialophora gregata. The minimum inhibitory concentration MIC value of the phialophora gregata is 32 mu g / mL, and the minimum bacteriocidal concentration MBC value is 64 mu g / mL.

Description

technical field [0001] The invention relates to a new application of the compound, clarifies the relationship between the chemical structure of the medicine and the biological activity of the bacteria, more specifically, explores the antibacterial and bactericidal activities of the compound on the soybean stem brown rot pathogen. Background technique [0002] Soybean stem brown rot is an important disease in soybean production, and the pathogen is Soybean stem brown rot. At present, soybean stem brown rot occurs in the midwestern and southeastern states of the United States, Canada, Brazil, Argentina, Mexico, Egypt, Japan, and the former Yugoslavia. [0003] The main characteristic of soybean stem brown rot damage is that the vascular bundles and pith of the soybean stem turn reddish brown, and then the whole stem turns brown, and it can cause leaf damage in the late growth period of soybean (usually in August) or under dry conditions. Discoloration and necrosis between lea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/86A01P3/00
Inventor 不公告发明人
Owner 许自协