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Molecular markers related to chicken feathering traits and their applications

A technology of molecular markers and fluffing, applied in the direction of DNA / RNA fragments, recombinant DNA technology, etc., can solve the problems of high financial resources, human burden, and prolonging the breeding cycle, so as to speed up the breeding process, shorten the breeding cycle, and improve the accuracy Effect

Inactive Publication Date: 2018-06-12
INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE JIANGXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This requires a large number of test crosses, which not only prolongs the breeding cycle, but also causes a high financial and human burden

Method used

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  • Molecular markers related to chicken feathering traits and their applications
  • Molecular markers related to chicken feathering traits and their applications
  • Molecular markers related to chicken feathering traits and their applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1, the specific primer design of the KRT gene linkage group A117G mutation site of chicken E22C19W28_E50C23 region chicken

[0035] Xiushui yellow-feathered black-bone chicken is a famous breed of veiled chicken in my country. Because the veiled gene is a dominant gene, there are still some heterozygotes carrying recessive genes in the population. The offspring produced between the homozygotes of the flipping gene, the flipping trait does not segregate, and they are all flipped. There will be about 1:1 homozygote and heterozygote for turning hair gene between the homozygote and the heterozygote for turning hair gene, so that the non-hair turning gene can be passed down. The offspring produced between the heterozygotes of the fluff gene will have flat-haired individuals.

[0036] 1. Discovery of SNP polymorphism

[0037] A SNP marker was found to be significantly associated with the moulting trait during sequencing. There is a base substitution of A56648-G5...

Embodiment 2

[0043]Example 2, PCR amplification and sequencing of the hair turning gene of Xiushui yellow-feathered black-bone chicken

[0044] 260 Xiushui yellow-feathered black-bone chickens: purchased from Xiushui County, Jiangxi Province.

[0045] 1. Genomic DNA extraction

[0046] Blood was collected from the wing vein of each sample, and the genomic DNA was extracted by the high-salt method.

[0047] The specific process is as follows:

[0048] (1) Configure the solution for the extraction of genomic DNA of the chicken to be tested

[0049] Solution A: 50mM Tris·Cl (pH 8.0), 5mM EDTA (pH 8.0), 20% SDS, stored at room temperature. Solution B: Chloroform: Isoamyl Alcohol (24:1), store in shading.

[0050] 1) Take 100μl chicken blood into a 1.5 mL centrifuge tube

[0051] 2) Add 600μL solution A to a 1.5 mL centrifuge tube.

[0052] 3) Add 10 μL of ProK (20 mg / mL) to each 1.5 mL centrifuge tube and mix thoroughly.

[0053] 4) Digest overnight in a water bath at 55°C.

[0054] 5)...

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Abstract

The invention discloses a molecular marker related to a chicken frizzled feather character and an application of the molecular marker, and belongs to the technical field of biology. An A56648-G56648 base substitution exists on a 56648th basic group of a KRT gene linkage group (genbank serial number AADN03009152.1) of a chicken in an E22C19W28_E50C23 area, and causes SNP polymorphism, and the chicken frizzled feather character is judged by detecting the base substitution condition of a primer on the site for chicken marker assisted selection correlation analysis.

Description

technical field [0001] The invention belongs to the technical field of molecular marker preparation in poultry genetic engineering, and in particular relates to a molecular marker related to chicken fluffing traits used as chicken marker-assisted selection association analysis and its application in association analysis. Background technique [0002] In recent years, with the development of molecular genetics and biotechnology, there have been more and more researches on chicken molecular biology. my country is a country that pays attention to gourmet food. The favor of chicken stems from the eating habits developed over thousands of years. The consumer market of poultry products is mainly fresh and live. It pays attention to the external characteristics of the products and requires the products to have a good appearance. Such as feather color, shape, etc. Although these traits have no direct economic value, due to the long-term consumption habits formed in different regions...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/6888
Inventor 武艳平谢金防霍俊宏刘林秀谢明贵康昭风季华员
Owner INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE JIANGXI ACAD OF AGRI SCI
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