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Maize rough dwarf virus variety resistance inoculation identification method established by taking wheat green dwarf virus plants as virus source

A technology of maize rough dwarf disease and identification method, which is applied in botany equipment and methods, horticultural methods, applications, etc., can solve problems such as poor accuracy, affecting the rate of SBPH infection, and limiting research time, so as to improve the accuracy of infection. The effect of increasing the rate and extending the identification time

Inactive Publication Date: 2016-01-20
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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AI Technical Summary

Problems solved by technology

Field identification is easily affected by factors such as environmental conditions, the occurrence of SBPH populations, and the number of migratory flights. The accuracy of identification is poor, so that the selection of disease-resistant varieties often requires repeated identification over many years, which greatly delays the resistance to coarse shrinkage of corn. disease breeding process
Artificial inoculation identification has improved the accuracy of identification of corn rough dwarf disease resistance. At present, the mature method is to inoculate the virus by feeding the brown planthopper carrying RBSDV on the diseased corn plants. The identification work has greatly limited the research time. In addition, SBPH cannot reproduce on corn, and using corn diseased plants as the source of virus will affect the rate of SBPH transmission

Method used

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  • Maize rough dwarf virus variety resistance inoculation identification method established by taking wheat green dwarf virus plants as virus source
  • Maize rough dwarf virus variety resistance inoculation identification method established by taking wheat green dwarf virus plants as virus source
  • Maize rough dwarf virus variety resistance inoculation identification method established by taking wheat green dwarf virus plants as virus source

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1, using the wheat green dwarf plant as the virus source to establish the resistance inoculation identification method of corn rough dwarf disease variety

[0016] (1) Acquisition of fresh wheat plants carrying RBSDV

[0017] In April 2013, fresh wheat plants showing typical symptoms of wheat green dwarf disease, such as dwarf plants, dark green leaves, slightly increased tillers, and notched heart leaves, were collected in Jianhu, Jiangsu, a serious disease area ( figure 1 ). The total RNA of wheat leaves was extracted according to the instructions of Trizol (Reagent). Using published primers RB1-S9-F: 5′-GRTAGACAGGCAAAYMTAAGCGT-3′ (R: A / G; Y: T / C; M: A / C) and RB-S9-R: 5′-GGATTACAACAHACACAMCGAAA-3′ (H: T / G; M: G / A / T) (primers were synthesized by Shanghai Sangon Biotechnology Co., Ltd.) for RT-PCR detection to determine whether the suspected wheat strains carry RBSDV. Using 2 μL of extracted wheat leaf total RNA as a template and RB-S9-R as a primer, rever...

Embodiment 2

[0022] Embodiment 2, the comparative study of taking wheat green dwarf disease plant and corn rough dwarf disease plant as poison source respectively

[0023] (1) Comparison of poisonous rate of SBPH

[0024] With reference to (2) in Example 1, utilizing wheat green dwarf plant and corn rough dwarf plant respectively as the source of poison to feed 1-2 ages of SBPH ( Figure 5 ), and after the cycle period, 100 SBPHs were collected to detect the toxin-carrying rate of SBPH fed by two kinds of toxin sources by Dot-ELISA method. The results showed that the virus-carrying rate of SBPH fed on wheat green dwarf plants was 35% ( Figure 6A ), while the rate of SBPH feeding on corn rough dwarf plants was 13% ( Figure 6B ).

[0025] (2) Comparison of maize disease incidence

[0026] With reference to (3) in Example 1, utilize the wheat green dwarf plant and the maize rough dwarf plant respectively to inoculate corn (the effective amount of inoculated worms is 3 heads / plant) with ...

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Abstract

The invention provides a maize rough dwarf virus variety resistance inoculation identification method established by taking wheat green dwarf virus plants as a virus source. The invention adopts the principle that wheat plants with wheat green dwarf virus classical symptoms are collected in a field at a jointing stage of wheat, wheat green dwarf virus plants are determined by using a molecular detection method, and maize rough dwarf virus resistance inoculation identification is carried out on maize varieties by taking wheat green dwarf virus plants as the virus source. By using the method provided by the invention to evaluate the resistance level of the maize varieties on maize rough dwarf viruses, the variety resistance identification time is shortened and the virus carried rate of laodelphax striatellus is increased. The method can be applied to the fields of discovery and identification of maize variety resources, variety resistance evaluation, disease-resistant variety breeding and resistance genetic rule research and other fields.

Description

Technical field: [0001] The invention relates to an inoculation identification method for corn rough dwarf variety resistance established by using wheat green dwarf plants as a poison source and its application in agriculture, belonging to the field of agricultural science and technology. Background technique: [0002] Rice blackstreakeddwarfvirus (RBSDV) belongs to the second group of Fijivirus in the family Reoviridae, and uses SBPH as the main vector for persistent transmission. Toxin, and can transmit virus continuously, but not through eggs. Under natural conditions, the main hosts are rice, corn, barley, wheat, sorghum, millet, arinaea, barnyardgrass and dogtail grass and other gramineous plants. Among them, artificial inoculation of corn is the most sensitive. In my country, RBSDV infects wheat to cause wheat green dwarf, infects maize to cause maize rough dwarf, and infects rice to cause rice black-streaked dwarf. Typical symptoms of wheat green dwarf plants are dw...

Claims

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Application Information

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IPC IPC(8): A01G7/00
CPCA01G7/00
Inventor 周彤杜琳琳周益军吕建颖曹晓燕兰莹孙枫
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES