Molecular marker of TaGS2 gene related with wheat plant height, obtaining method and application thereof

A molecular marker and high-correlation technology, which is applied in biochemical equipment and methods, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of photosynthetic efficiency and yield reduction, poor ventilation and light transmission in the middle and lower parts, and easy lodging, etc. problems, to achieve the effect of improving selection efficiency and quality, fast and accurate screening, and improving quality and output

Active Publication Date: 2015-08-19
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Plant height is an important factor affecting the high yield of wheat. Plants that are too tall are prone to lodging under high fertilizer and water conditions, resulting in a decrease in yield; plants that are too short can easily cause crowded leaves, poor ventilation and light transmission in the middle and lower parts, resulting in reduced photosynthetic efficiency and yield.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 Relevant with wheat plant height TaGS2 Acquisition of molecular markers for genes

[0032] 1. Wheat Konon 9204 TaGS2 gene cloning

[0033] Design primers according to the results of database analysis:

[0034] Nucleotide sequence of upstream primer: 5'-TCCTCCCTCGTCTCGTCCGCGT-3' (SEQ ID NO: 3)

[0035] Nucleotide sequence of downstream primer: 5'-AGTGCCCCGACGGAACCACAGG-3' (SEQ ID NO: 4)

[0036] Total RNA was extracted from the three-leaf stage seedlings of hexaploid wheat Kenong 9204, and 1284bp full-length cDNAs of TaGS2-A, TaGS2-B and TaGS2-D coding genes were amplified by RT-PCR.

[0037] The specific operation process is as follows:

[0038] 1) Plant total RNA extraction: Grind 100 mg of wheat Konon 9204 seedlings with liquid nitrogen, transfer to an EP tube containing 1 mL of Trizol reagent after grinding, mix well, and let stand at room temperature for 5 min; add 0.2 mL of chloroform, fully Mix well and let stand at room temperature for 2-3 min;...

Embodiment 2

[0062] Example 2 Application of Molecular Marker IN10 in Detection and Screening of Wheat Varieties or Strains

[0063] (1) Use IN10 labeled primers (upstream primer nucleotide sequence such as SEQ ID NO: 1, downstream primer nucleotide sequence such as SEQ ID NO: 2) to F 6 The DNA of 188 derivative lines of the generation RIL population was amplified by PCR respectively. The PCR amplification system was 20 μl, including: 10 μl of 2×Taq PCR MasterMix, 1 μl of upstream and downstream primers, 1 μl of DNA template of the wheat to be tested, and the rest with ddH 2 O supplementation; PCR amplification program: pre-denaturation at 94°C for 5 min; denaturation at 94°C for 30 s, annealing at 56°C for 30 s, extension at 72°C for 1 min / kb, 30 cycles; extension at 72°C for 7 min; storage at 20°C ;

[0064] (2) Put the above amplification products on 6% non-denaturing polyacrylamide gel (6% non-denaturing polyacrylamide gel means that 100ml polyacrylamide gel solution contains 5.85 g ...

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Abstract

The invention discloses a molecular marker IN10 of a TaGS2 gene related with the wheat plant height, and the application in the field of screening wheat variety or strain plant height by using the molecular marker; in particular, a marker primer of the molecular marker IN10 is used for performing PCR (polymerase chain reaction) amplification on a wheat genome DNA (deoxyribonucleic acid) to be tested, and the product of amplification is subjected to electrophoresis separation; the obtained corresponding product of amplification has bands in 555bp, 466bp and 426bp of molecular weights, and is used for judging the change of plant height of the variety, which is used as a theoretical guide for breeding new wheat variety of different plant height types. The screening method provided by the invention is quick and accurate and is not affected by environment, the selection target is definite, a reasonable plant height group can be quickly produced, and the selection efficiency and quality of high-yield wheat variety or strain can be improved greatly.

Description

technical field [0001] The invention relates to the technical field of biogenetic engineering, in particular to a wheat plant height-related TaGS2 Gene molecular markers, acquisition methods and their applications. Background technique [0002] Wheat is the most important food crop in the world and one of the three major food crops in my country. Plant height is an important factor affecting the high yield of wheat. Plants that are too tall are prone to lodging under high fertilizer and water conditions, resulting in a decrease in yield; plants that are too short can easily cause crowded leaves, poor ventilation and light transmission in the middle and lower parts, resulting in reduced photosynthetic efficiency and yield. From the 1960s to the 1980s, the promotion of dwarf and semi-dwarf wheat varieties played a key role in increasing wheat production worldwide. In addition to being controlled by the main dwarf gene, the plant height traits of wheat are also characterized ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张玮李俊明赵学强樊小莉纪军崔法
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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