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A kind of Zoupi papaya extract, extraction method and application thereof

An extraction method and extract technology, applied in the field of medicine, can solve the problems of many side effects and high recurrence rate, and achieve the effects of less toxic and side effects, lowering the ulcer index, and increasing the ulcer inhibition rate

Active Publication Date: 2019-07-09
CHINA THREE GORGES UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the drugs for treating gastric ulcer mainly include gastric acid inhibitors, histamine receptor antagonists, proton pump inhibitors, Helicobacter pylori inhibitors, gastric mucosal protective agents, gastric motility drugs, etc. The use of the above drugs has an average effect on the recent ulcer healing rate. Higher, but the recurrence rate is high after drug withdrawal, and the problem of many side effects is still difficult to solve

Method used

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  • A kind of Zoupi papaya extract, extraction method and application thereof
  • A kind of Zoupi papaya extract, extraction method and application thereof
  • A kind of Zoupi papaya extract, extraction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Papaya total triterpenoids, a triterpene component with the effect of treating gastric ulcer in wrinkled papaya, are specifically prepared by the following method:

[0029] 1) Pretreatment of wrinkled papaya

[0030] The near-ripe fruit of wrinkled papaya is washed, sliced ​​and dried in the sun, and 0.005 kg is taken for preservation, and the rest is dried in a constant temperature blower box at 48°C to constant weight, with a net weight of 5.0 kg.

[0031] 2) Depigmentation of wrinkled papaya

[0032] The sample was pulverized with a pulverizer, crossed a 20-mesh sieve, and used sherwood oil as a solvent at a liquid-solid ratio of 6:1, decolorized 3 times at 60° C. for 2 hours each time, and obtained 4.26 kg of papaya coarse powder after decolorization (see figure 1 ).

[0033] 3) Reflux extraction of papaya coarse powder after decolorization

[0034] The decolorized papaya coarse powder obtained in the above 2) was extracted with ethyl acetate as a solvent at a liqu...

experiment example 2

[0045] In the following, in conjunction with specific experimental examples, it is specifically illustrated that the total triterpenes of papaya and oleanolic acid obtained in Example 1 of the present invention have an obvious preventive effect on gastric ulcers in mice induced by water immersion restraint stress.

[0046] experiment method

[0047] 1) Experimental animals

[0048] Animals and feed were purchased from the Experimental Animal Center of China Three Gorges University, SPF-grade Kunming mice, weighing 18-22g, half male and half male, experimental animal quality certificate number: SCXK (E) 2011-0012; animals were kept in a normal-grade animal room and dried , ventilated, quiet environment, 10 / cage.

[0049] 2) Grouping and administration

[0050] 90 Kunming mice were randomly divided into 9 groups: blank control group, ulcer model group, low, medium and high dose groups of papaya total triterpenes (25, 50 and 100 mg / kg), low, medium and high dose groups of olean...

experiment example 3

[0071] Below in conjunction with specific experimental examples, it is specifically illustrated that the total triterpenes of papaya and oleanolic acid obtained in Example 1 of the present invention have obvious preventive effects on ethanol-induced gastric ulcers in mice.

[0072] experiment method

[0073] 1) Experimental animals

[0074] Animals and feed were purchased from the Experimental Animal Center of China Three Gorges University, SPF-grade Kunming mice, weighing 18-22g, half male and half male, experimental animal quality certificate number: SCXK (E) 2011-0012; animals were kept in a normal-grade animal room and dried , ventilated, quiet environment, 10 / cage.

[0075] 2) Grouping

[0076] 144 Kunming mice were randomly divided into 9 groups, 16 in each group: blank control group, ulcer model group, low, medium and high dose groups (25, 50 and 100 mg / kg) of papaya total triterpenes, low oleanolic acid , middle and high dose groups (25, 50 and 100mg / kg) and ranitid...

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Abstract

The invention discloses a chaenomeles speciosa (Sweet) Nakai extractive, and an extraction method and application thereof. Concretely, the extractive is chaenomeles speciosa (Sweet) Nakai total triterpenes and oleanolic acid separated from chaenomeles speciosa (Sweet) Nakai total triterpenes, and the extractive is applied to prepare medicine for preventing and treating gastric ulcer diseases. The extraction method comprises the following concrete steps: decoloring chaenomeles speciosa (Sweet) Nakai by using petroleum ether; then performing refluxing extraction on decolored chaenomeles speciosa (Sweet) Nakai by using ethyl acetate; then performing two times of decoloring, in other words, decoloring a chaenomeles speciosa (Sweet) Nakai extractum by using petroleum ether and then decoloring again by using active carbon; merging filtrates, and recovering ethanol, so as to obtain chaenomeles speciosa (Sweet) Nakai total triterpenes; and finally, performing recrystallization on the extracted chaenomeles speciosa (Sweet) Nakai total triterpenes for two times by using anhydrous ethanol, so as to obtain colorless oleanolic acid. The method is simple, and the product purity is high. The obtained chaenomeles speciosa (Sweet) Nakai total triterpenes and oleanolic acid both possess relatively good prevention and treatment effects, possess the effect better than that of ranitidine, also possess the advantages of small toxic and side effects and relatively good curative effect, and is worth exploiting and applying.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the extraction of papaya total triterpenes in wrinkled papaya, the separation and purification method of oleanolic acid and its application in treating gastric ulcer. Background technique [0002] Wrinkled papaya is the dry and near-ripe fruit of Chaenomeles speciosa (Sweet) Nakai, a plant of the Rosaceae family. Papaya, known as the "fruit of all benefits", is a very important ornamental and edible plant, and was listed as one of the first batch of medicinal and edible foods recognized by the Ministry of Health in 2003. It is used as an edible fruit, and is also used in the field of beauty cosmetics and health care products. At the same time, as a commonly used traditional Chinese medicine, it tastes sour and warm, enters the liver and spleen meridians, and has the functions of calming the liver, relaxing tendons, and harmonizing the stomach. The effect of dampness...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/732A61K31/56A61P1/04
Inventor 贺海波覃慧林石孟琼张永峰刘呈雄汪鋆植周琴程凡邹坤张继红
Owner CHINA THREE GORGES UNIV
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