Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of medium-term preservation solution of cornea and preparation method thereof

A technology of preservation solution and cornea, which is applied in the preservation, application, and animal husbandry of human or animal bodies, and can solve the problems of impossibility of penetrating cornea transplantation, reduction, etc., achieve less cell fusion, enhance survival ability, and avoid Effects of endothelial cell apoptosis

Active Publication Date: 2017-11-07
XIAMEN UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many studies have shown that the number of viable corneal endothelial cells will gradually decrease with the prolongation of storage time, so that they cannot be used for penetrating keratoplasty. Therefore, it is necessary to provide a medium-term corneal fluid that can improve the activity of corneal endothelial cells and prolong storage time.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of medium-term preservation solution of cornea and preparation method thereof
  • A kind of medium-term preservation solution of cornea and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A medium-term corneal preservation solution, which is a MEM cell culture medium containing chondroitin sulfate, low-molecular dextran, L-glutamine, fetal bovine serum, tobramycin, Hepes and rapamycin, Wherein the concentration of each component is as follows: chondroitin sulfate 25g / L, low molecular weight dextran 10g / L, L-glutamine 0.375mg / L, fetal bovine serum 5%, tobramycin 0.1g / L, Hepes9. 5g / L, rapamycin 1nM / L.

[0028] The preparation method of above-mentioned medium-term preservation solution of cornea comprises:

[0029] 1) According to the instructions of the MEM medium, take 9.4g of MEM medium powder and add 500ml of water for injection, dissolve and then filter and sterilize to prepare the MEM medium;

[0030] 2) Take 25g of chondroitin sulfate and add 200ml of water for injection, dissolve and filter to sterilize to prepare a chondroitin sulfate solution of 125g / L; take 10g of low-molecular dextran (average molecular weight 20000-40000kD) and add 100ml of wa...

Embodiment 2

[0035] In this embodiment, the concentration of each component in the medium-term corneal preservation solution is as follows: 21 g / L of chondroitin sulfate, 9 g / L of low molecular weight dextran, 0.372 mg / L of L-glutamine, and 3% of fetal bovine serum , Tobramycin 0.09g / L, Hepes 9.2g / L, rapamycin 0.95nM / L. All the other are with embodiment 1.

Embodiment 3

[0037] In this embodiment, the concentration of each component in the medium-term corneal preservation solution is as follows: chondroitin sulfate 24g / L, low molecular weight dextran 11g / L, L-glutamine 0.378mg / L, fetal bovine serum 4% , Tobramycin 0.11g / L, Hepes 9.8g / L, rapamycin 1.05nM / L.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a medium-term corneal preservation solution and a preparation method thereof. The medium-term corneal preservation solution contains chondroitin sulfate, low-molecular dextran, L-glutamine, fetal bovine serum, tobramycin, Hepes and Rapa Mycin MEM cell culture medium, the concentration of each component is as follows: chondroitin sulfate 20-26g / L, low-molecular dextran 8-12g / L, L-glutamine 0.37-0.38mg / L, fetal bovine serum 2-5.5%, tobramycin 0.08-0.12g / L, Hepes 9-10g / L, rapamycin 0.9-1.1nM / L. The mid-term corneal preservation solution of the invention can protect corneal endothelial cells, avoid endothelial cell necrosis and fusion phenomena caused by long-term preservation, and prolong corneal preservation time more effectively.

Description

technical field [0001] The invention belongs to the technical field of corneal preservation solution preparation, and in particular relates to a corneal medium-term preservation solution and a preparation method thereof. Background technique [0002] In recent years, corneal disease has become the second leading cause of blindness in my country. However, as a curable eye disease, patients can regain their light by performing lamellar or penetrating keratoplasty. According to the preservation time of endothelial cell activity, corneal preservation can be divided into short-term, medium-term and long-term preservation. Mid-term preservation is a method of maintaining the activity of corneal endothelial cells for more than 4 days by keeping the preservation solution at 4°C. It has become the corneal preservation method widely used by eye banks in the world. [0003] The corneal endothelium is a single layer of cells inside the cornea, which can continuously drain the water in...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 李程刘昭麟吴洁丽刘祖国
Owner XIAMEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com