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Application of Chenopodolin in preparation of medicines for treating ovarian cancer

A kind of chenopodolin, 1. The technology of chenopodolin, which is applied in the application field of chenopodolin in the preparation of drugs for treating ovarian cancer, can solve the problems that there is no report of ovarian cancer activity, no antibacterial or antifungal activity, etc.

Inactive Publication Date: 2016-02-03
吴正锋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that the compound is phytotoxic, but has not exhibited any antibacterial or antifungal activity
[0003] There is no report on the activity of this compound on ovarian cancer

Method used

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  • Application of Chenopodolin in preparation of medicines for treating ovarian cancer
  • Application of Chenopodolin in preparation of medicines for treating ovarian cancer
  • Application of Chenopodolin in preparation of medicines for treating ovarian cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Example 1: Isolation, Preparation and Structure Confirmation of Chenopodolin

[0011] The preparation method of Chenopodolin is the same as that reported in the literature (Chenopodolin: APhytotoxic Unrearranged ent-Pimaradiene Diterpene Produced by Phomachenopodicola, a Fungal Pathogen for Chenopodium album Biocontrol, J. Nat. Prod., 2013, 76, 1291-1297).

[0012] Structural confirmation: the molecular formula is C 24 h 30 o 8 , with an unsaturation of 10. H NMR spectrum data δ H (ppm, DMSO-d 6 , 600MHz): H-1 (5.00, d, J=4.8), H-2 (4.62, br, d, J=4.8), H-3 (4.28, br, s), H-5 (2.74, s ), H-7 (5.85, s), H-9 (2.85, m), H-11 (1.50, m), H-12 (4.56, dd, J=10.0, 4.1), H-14 (2.62, d, J=16.2), H-14 (2.26, d, J=16.2), H-15 (5.79, dd, J=17.8, 11.0), H-16 (5.17, d, J=11.0), H- 16 (4.97, d, J=17.8), H-17 (1.06, s), H-19 (1.48, s), H-20 (1.08, s), 1-COMe (2.24, s), 12-COMe (2.12, s); carbon NMR spectrum data δ C (ppm, DMSO-d 6 , 600Hz): 71.8 (CH, 1-C), 79.3 (CH, 2-C), 76.8...

Embodiment 2

[0013] Embodiment 2: Pharmacological action test of Chenopodolin

[0014] 1. Materials and Instruments

[0015] The human ovarian serous papillary cystadenocarcinoma cell line SKOV3 was provided by the Medical Experimental Center of Lanzhou University. Common RPMI-1640 medium (10% fetal bovine serum) culture. Chenopodolin is self-made, and the HPLC normalized purity is greater than 98%. RPMI-1640 culture medium, tetramethylazoblue (MTT), dimethyl sulfoxide (DMSO), L-glutamine Kehao Bioengineering Co., Ltd. Trypsin was purchased from Sigma, USA. Fetal bovine serum was purchased from Hangzhou Sijiqing Bioengineering Materials Co., Ltd. Sodium dodecyl sulfonate (SDS) was purchased from Xi'an Zhouding Biotechnology Co., Ltd. HER2 (FITC marker) was purchased from Beijing Boaosen Biotechnology Co., Ltd. Acidified HER2 (FITC-labeled) was purchased from Beijing Boaosen Biotechnology Co., Ltd. Penicillin and streptomycin were purchased from Huabei Pharmaceutical Factory.

[001...

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Abstract

The invention discloses an application of Chenopodolin in preparation of medicines for treating an ovarian cancer, and belongs to the field of medicines. An in vitro test proves that the Chenopodolin is capable of inhibiting proliferation activity of an ovarian cancer SKOV3 cell; an MTT result shows that distribution of a cell cycle is affected by the Chenopodolin; tumor cells are retarded at a G0 / G1 stage; meanwhile, the Chenopodolin can be combined with a tyrosine kinase phosphorylation ATP binding site of an EGFR / HER2 acceptor to inhibit phosphorylation of tyrosine kinase, so that cell apoptosis is induced by down-regulating expression of HER2 and P-HER2 protein; and the Chenopodolin can be further researched and developed into the medicines for treating the ovarian cancer.

Description

technical field [0001] The invention relates to a new application of the compound Chenopodolin, in particular to the application of the Chenopodolin in the preparation of drugs for treating ovarian cancer. Background technique [0002] AlessioCimmino and others isolated and purified the compound Chenopodolin for the first time, and published the results in the famous Journal of Natural Products (Chenopodolin: APhytotoxic Unrearrangedent-Pimaradiene Diterpene Produced by Phomachenopodicola, a FungalPathogenforChenopodiumalbumBiocontrol, J.Nat.Prod., 2013, 1-1-2979). Studies have shown that the compound is phytotoxic, but has not exhibited any antibacterial or antifungal activity. [0003] There is no report about the activity of this compound on ovarian cancer so far. Contents of the invention [0004] The object of the present invention is to provide a medical application of Chenopodolin. [0005] The above-mentioned purpose is achieved through the following technical so...

Claims

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Application Information

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IPC IPC(8): A61K31/365A61P35/00
Inventor 吴正锋
Owner 吴正锋
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