A symbiotic bacteria system and its application to improve the biomass and oil quality of Scenedesmus obliquus

A technology for Scenedesmus obliquus and symbiotic bacteria, which is applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of culture system collapse, pollution, low yield, etc.

Active Publication Date: 2019-03-15
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current yield of this species of algae is low, and its optimum culture pH is 7-8. Microalgae at neutral pH are extremely susceptible to contamination by foreign species, leading to the collapse of the culture system.

Method used

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  • A symbiotic bacteria system and its application to improve the biomass and oil quality of Scenedesmus obliquus
  • A symbiotic bacteria system and its application to improve the biomass and oil quality of Scenedesmus obliquus
  • A symbiotic bacteria system and its application to improve the biomass and oil quality of Scenedesmus obliquus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Screening and identification of Scenedesmus obliquus symbiotic bacteria

[0047] The screening and identification method of Scenedesmus symbiotic bacteria in the present invention comprises the following steps:

[0048] 1. Purification

[0049] Scenedesmus obliquus used in the present invention can be any strain of Scenedesmus obliques known in the art. Scenedesmus obliquus used in this example comes from a freshwater lake and has been continuously subcultured in the laboratory for three years.

[0050] Inoculate 5 mL of Scenedesmus obliquus culture solution into 1 L of fresh sterilized BG11 medium, culture for 14 days, and take the Scenedesmus culture solution on the 4th day and the 14th day respectively. Dilute 10 with ultrapure water 4 -10 6 times, take 100 microliters to coat YPD and inorganic salt medium respectively, put them in an incubator, and incubate at 27°C for 3 days. Single colonies formed in different plates were streak purified.

[0051] ...

Embodiment 2

[0180] Example 2 Effects of Scenedesmus obliquus, symbiotic bacteria and their mixed flora co-cultivation on the growth of Scenedesmus obliquus

[0181] Inoculate the screened single species of symbiotic bacteria and their mixed flora (Table 1) and Scenedesmus obliquus at a ratio of 1:3 (number / number) to a sterilized 1L container (121°C, 20 minutes) In a cylindrical photobioreactor (50 cm in height and 6.5 cm in diameter) in BG11 medium, a co-cultivation system of Scenedesmus obliquus and bacteria was constructed. The inoculation conditions are: the inoculated Scenedesmus obliques and symbiotic bacteria are taken from the logarithmic growth phase; the culture temperature is 22-30 ° C, the optimum is 27 ° C; air and carbon dioxide (air 0.2 L / min, carbon dioxide 5mL / min), the light intensity is 120μmolm -2 the s -1 , continuous light.

[0182] The experimental results show (as in Table 2, image 3 ), after 10 days of cultivation, in the co-cultivation system of symbiotic ba...

Embodiment 3

[0186] Example 3 Effect of Scenedesmus obliquus, symbiotic bacteria and their mixed flora co-cultivation on oil production of Scenedesmus obliquus

[0187] Inoculate the screened single species of symbiotic bacteria and their mixed flora (Table 1) and Scenedesmus obliquus at a ratio of 1:3 (number / number) into a 1 L sterile (121°C, 20 minutes) BG11 culture In a base cylindrical photobioreactor (50 cm high, 6.5 cm in diameter), a co-culture system of Scenedesmus obliquus and bacteria was constructed. The inoculation conditions are: the inoculated Scenedesmus obliques and symbiotic bacteria are taken from the logarithmic growth phase; the culture temperature is 22-30 ° C, the optimum is 27 ° C; air and carbon dioxide (air 0.2 L / min, carbon dioxide 5mL / min), the light intensity is 120μmol m -2 the s -1 , continuous light.

[0188] The oil analysis method used is as follows: Take Scenedesmus 3d, 6d, and 9d during the cultivation process of Scenedesmus, weigh 20 mg after freeze-...

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Abstract

The invention relates to the field of micro-alga biotechnologies, in particular to a symbiotic bacterium system for increasing biomasses of scenedesmus obliquus and improving the quality of grease and the stability of culture systems and application of the symbiotic bacterium system. The symbiotic bacterium system comprises the scenedesmus obliquus and symbiotic bacteria. The symbiotic bacteria include one type or a plurality of types of brevunmdimonas sp., rhizobium sp., pseudomonas sp. and acidovorax sp. The symbiotic bacterium system and the application have the advantages that the symbiotic bacteria and mixed floras of the symbiotic bacteria are applied to scenedesmus obliquus culture procedures, extracellular substances and oxygen which are generated by the scenedesmus obliquus can be consumed by each single type of symbiotic bacteria and the mixed floras, carbon dioxides can be generated to be utilized by the scenedesmus obliquus, micro-environments favorable for growth of the scenedesmus obliquus can be created, and accordingly substance exchange and metabolism of the scenedesmus obliquus, the symbiotic bacteria and the mixed floras can be promoted.

Description

technical field [0001] The invention relates to the field of microalgae biotechnology, in particular, the invention relates to a symbiotic bacteria system for improving the biomass of Scenedesmus obliquus, the quality of oil and the stability of a culture system and its application. Background technique [0002] With the decrease of fossil energy and the serious environmental problems it brings, it is urgent to find alternative energy. Microalgae is considered to be the most promising third-generation biomass energy to replace fossil energy, but the high cost of cultivation has become a bottleneck for its development. Outdoor large-scale cultivation is an important means to reduce the cost of microalgae cultivation, but the biomass of outdoor cultured microalgae is very low and is easily polluted by other species. Especially for oil-producing microalgae with a neutral pH, the contamination of other species can easily lead to the collapse of the culture system, and the cost ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/12C12P7/64C12R1/89C12R1/01C12R1/41
Inventor 丛威王瑞民颜成虎薛升长
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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