Engineering bacterium, construction method and application for producing immobilized alkaline pectinase nano micro-spheres

Abstract

The invention discloses an engineering bacterium and a construction method for producing alkaline pectinase immobilized nano micro-spheres by the aid of one-step processes, and belongs to the technical field of immobilized enzymes. A recombinant Escherichia coli strain E.coli BL21 lambda (DE3) pABC-PGL constructed by the aid of the gene recombination method is preserved in the General Biology Center of the China Committee for Culture Collection of Microorganisms, and a strain preservation number of the recombinant Escherichia coli strain is CGMCC NO.10911. The engineering bacterium and the construction method for producing the immobilized alkaline pectinase nano micro-spheres on the basis of the Escherichia coli strains have the advantages that alkaline pectinase genes which are currently industrially widely applied are fused at N tail ends of PHA (phytohemagglutinin) synthesis enzyme genes phaC by connecting peptides by the aid of the gene fusion method, recombinant gene segments are transformed into recombinant bacteria to be expressed in an inducible manner, nano micro-sphere complexes can be synthesized in the recombinant bacteria, alkaline pectinase is carried on the surfaces of the nano micro-sphere complexes and can be effectively combined with the surfaces of the nano micro-spheres to form immobilized alkaline pectinase, and alkaline pectinase immobilization production can be implemented at one step.

Description

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AI Technical Summary

Benefits of technology

In this new technology, there're two main benefits - firstly, avoiding complicated processes for producing large quantities of acidic proteases like lysozolinum (which causes skin rash), which makes them expensive but also harmful when ingested over time due to their ability to break down protein molecules). Secondly, creation of an effective way to attach these small particles onto surfaces without causing damage during manufacturing process. These techniques allow for precise control over how well different types of microorganisms produce certain substances called alkali metal chelates.

Problems solved by technology

This patented problem addressed in this patents relates to improving the performance and effectiveness of aluminium salt hydrolases such as alkali metal lipocaprilyl lactone sulfonatrylate lysochloroaccharides (ALCL). Immobilizing these enzymas onto carriers made of various materials helps reduce costs while maintaining their effects over long periods of storage. Additionally, combining them together allows for easier handling during manufacturing processes without losing valuable components.

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