A kind of primer and rapid detection method for detecting Atractylodes rhizome root rot
A root rot pathogen and detection method technology, which is applied in the field of primers and rapid detection for the detection of Atractylodes rhizome root rot, can solve the problems that cannot meet the needs of accurate plant protection work, rapid prevention and control, rapid spread of Atractylodes rhizome root rot, and delay in prevention and control opportunities, etc. Achieve the effects of improving timeliness and accuracy, less interference, and improving sensitivity
Active Publication Date: 2018-08-31
RES INST OF TRADITIONAL CHINESE MEDICINE PLANTS HUBEI ACAD OF AGRI SCI
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Problems solved by technology
The identification of traditional pathogenic bacteria can only be carried out after the plants show symptoms. It requires the isolation of pathogenic bacteria, purification and culture, verification of Koch's law, morphological observation, physiological and biochemical traits, etc. to identify. If it goes well, it will take about 10-20 days to complete the whole process , but once other bacteria contaminate the medium, it will affect the identification speed, and at the same time, the long-term identification will delay the timing of prevention and control
The shortcomings of traditional identification, such as inaccurate identification, time-consuming, and laborious, can not meet the requirements of precise and rapid prevention and control of plant protection work.
In order to solve the current situation of the rapid spread of Atractylodes rhizome root rot, and to achieve the purpose of rapid and accurate identification of Atractylodes rhizome caused by Atractylodes spp. Detection method of Atractylodes rhizome root rot
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Embodiment 1
[0029] A kind of Atractylodes macrocephala root rot detection primer and rapid detection method of the present invention comprise the following steps:
[0030] (1) Sampling and genomic DNA extraction:
[0031] In April 2014, 7 Atractylodes macrocephala seedlings infected with root rot and with obvious symptoms of root rot were randomly collected at the Atractylodes macrocephala planting base in Xiaocun Township, Xianfeng County, Enshi Tujia and Miao Autonomous Prefecture, and 7 Atractylodes atractylodes seedlings with no obvious root rot symptoms in the infected field were randomly collected, and then Number the samples in the order of 5-20, clean the root soil, rinse with sterile water 3 times, dry naturally, and then extract the sample DNA according to the CTAB method;
[0032] (2) The first round of amplification:
[0033] Using 5-11 sample DNA as a template, the first round of PCR amplification products were obtained through PCR amplification using the universal primers I...
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The invention relates to a primer and a rapid detection method for detecting Atractylodes rhizome root rot, which is characterized in that it comprises the following steps: (1) sampling; (2) first-round amplification; (3) second-round amplification; (4) Electrophoretic detection. A kind of Atractylodes rhizome root rot detection primer and rapid detection method of the present invention solves the current situation of the rapid spread of Atractylodes rhizome root rot, realizes the purpose of fast and accurate identification of Atractylodes rhizome root rot caused by Ceratobacteriaceae, and establishes a method capable of The detection method of Atractylodes rhizome root rot caused by the sclerobacteria to achieve the goals of "fast" and "accurate" effectively improves the timeliness and accuracy of detecting the root rot of Atractylodes rhizome caused by the sclerotium.
Description
technical field [0001] The invention relates to a primer for detecting Atractylodes macrocephala root rot and a rapid detection method. Background technique [0002] Atractylodes macrocephala (Atractylodes macrocephala) belongs to the family Asteraceae and is a perennial herb of the genus Atractylodes. It is mainly distributed in mountainous wetlands such as Hubei, Sichuan, Yunnan, Guizhou, and Zhejiang. The rhizome is used as medicine, which has the effects of invigorating the spleen and replenishing qi, drying dampness and promoting diuresis, antiperspirant, and antifertility. Bulk medicinal materials. You et al reported in "Plant Disease" in 2013 that the root rot of Atractylodes macrocephala caused by Ceratobasidium.sp is the biggest problem in the cultivation of Atractylodes macrocephala nationwide. The incidence rate is as high as 70%, and some fields even have no harvest. The occurrence and spread of Atractylodes rhizome root rot seriously affects the development a...
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IPC IPC(8): C12Q1/6895C12Q1/04C12N15/11C12R1/645
Inventor 游景茂林先明郭杰郭晓亮艾伦强孙扬柳
Owner RES INST OF TRADITIONAL CHINESE MEDICINE PLANTS HUBEI ACAD OF AGRI SCI