Passivating agent for reducing activity of Pb and Cr in alkaline soil and application thereof

A passivating agent and soil technology, applied in the field of microorganisms, can solve the problems of high cost of heavy metal pollution remediation technology, difficult production practice, single remediation agent, etc., and achieve the effects of wide economic benefits, maintaining soil ecological balance, and enhancing soil fertility.

Active Publication Date: 2016-02-10
湖南瑞丰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Existing restoration technologies for heavy metal pollution in alkaline farmland soil are high in c

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Preparation of Bacillus amyloliquefaciens bacteria powder:

[0057] Bacillus amyloliquefaciens strains were purchased from China Industrial Microorganism Culture Collection Management Center, numbered CICC23921, and the form was freeze-dried powder.

[0058] (1) Activation of slant strains

[0059] The slant of the test tube was nutrient agar medium, and cultured at 37°C for 20h.

[0060] (2) Shake flask culture

[0061] The liquid medium was cultured with beef peptone liquid, 37°C, 12h, 180r / min, and the liquid volume was 30ml / 250mL.

[0062] (3) 200L seed tank cultivation

[0063] The liquid medium is corn flour 1%, molasses 2%, yeast extract 0.2, MgSO 4 ·7H 2 O0.1g / L, K2HPO40.2g / L; inoculum size 0.35%, 37°C, aeration ratio 1:0.5, cultivated for 24h.

[0064] (4) Shallow tray fermentation culture in the fermentation bed

[0065] The solid medium adopts bran straw powder medium, bran and straw powder are mixed at a ratio of 5:1 (mass ratio), and then water is ad...

Embodiment 2

[0070] Preparation of Bacillus thuringiensis bacterial powder:

[0071] Bacillus thuringiensis strains were purchased from China Agricultural Microorganism Culture Collection and Management Center, numbered ACCC03343, and the form was freeze-dried powder.

[0072] (1) Activation of slant strains

[0073] The slant of the test tube is beef extract peptone medium, cultivated at 34°C for 20-24h.

[0074] (2) Shake flask culture

[0075] The liquid medium was cultured with beef peptone liquid, 34°C, 15h, 200r / min, and the liquid volume was 35ml / 250mL.

[0076] (3) 200L seed tank cultivation

[0077] The liquid medium is 3% soybean meal, 0.5% brown sugar, 0.25% starch, MgSO 4 ·7H 2 O0.5g / L, MnSO 4 0.15%, K 2 HPO 4 0.2g / L, CaCO 3 0.01%; inoculum size 0.2%-0.3%, cultured for 20 hours at 34°C with an aeration ratio of 1:0.5.

[0078] (4) Solid-state fermentation in ventilated fermentation tank

[0079] The solid medium adopts bran and rice husk medium. The bran and rice hus...

Embodiment 3

[0085] Recipe 1:

[0086] Bacillus amyloliquefaciens powder (the number of viable bacteria is 500 million / g, purchased from Shandong Xinchao Agricultural Technology Co., Ltd.) 420Kg;

[0087] Coffee grounds powder (fineness ≥ 80%, purchased from Shandong Today Coffee Co., Ltd.) 600Kg

[0088] Cordyceps militaris medium residue powder (fineness ≥ 80%, purchased from Shandong Dezhou Oriental Biotechnology Research Institute) 480Kg;

[0089] Blueberry pomace powder (fineness ≥ 80%, purchased from Daxinganling Baisheng Blueberry Technology Development Co., Ltd.) 840Kg;

[0090] Pomegranate pomace (fineness ≥ 80%, purchased from Shandong Permer Ponica Juice Co., Ltd.) 480Kg.

[0091] Preparation:

[0092] Horizontal twin-shaft paddle mixer model SSHJ-7 (purchased from Jinan Yanyi Machinery Equipment Co., Ltd.).

[0093] Adjust the mixer, enter the standby mode, start the machine, and mix the formula amount of Bacillus amyloliquefaciens powder, coffee grounds powder, Cordyceps m...

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PUM

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Abstract

The invention discloses a passivating agent for reducing the activity of Pb and Cr in alkaline soil and application thereof, and belongs to the field of heavy metal soil remediation. The passivating agent is prepared from, by weight, 5-20 parts of a microorganism bacterium agent, 10-30 parts of pomegranate pomace, 15-35 parts of coffee grounds, 5-30 parts of cordyceps militaris culture medium residues and 10-35 parts of blackberry pomace. Compared with the prior art, the passivating agent can significantly treat the alkaline farmland soil which is slightly polluted by plumbum and chromium, is low in cost and easy to operate and manage and has the very good application and popularization value.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a passivator for reducing the activity of Pb and Cr in alkaline soil and its application. Background technique [0002] The 2014 National Soil Pollution Survey Bulletin shows that heavy metal pollution in my country's farmland is mainly concentrated in chromium, cadmium, lead, arsenic, mercury, etc., and the pollution exceeding the standard is mainly mild and slight, and the proportion of heavy pollution is relatively small. . [0003] At present, for heavy metal pollution in farmland, domestic and foreign treatment methods mainly include in-situ application of lime, phosphate, silicate, clay minerals, chitosan for passivation treatment, and addition of biochar, planting hyperaccumulator plants, etc. and other technical means of governance. Ectopic restoration techniques include guest soil, electrodynamic restoration, rinsing, and composting and fermentation. These current technol...

Claims

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Application Information

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IPC IPC(8): C09K17/00A01N65/08A01P1/00A01P3/00B09C1/08C09K109/00C09K101/00
Inventor 常钟杨晓梅钱朋智周波周志强李在贞史晓洁蔡洋赵传涛侯大彬
Owner 湖南瑞丰生物科技有限公司
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